Original Papers
25 September 2013
Vol. 57 No. 3 (2013)
https://doi.org/10.4081/ejh.2013.e28

Continuous cyclic mechanical tension increases ank expression in endplate chondrocytes through the TGF-β1 and p38 pathway

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Continuous cyclic mechanical tension (CCMT), Endplate chondrocytes, ank, TGF-β1, p38.
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Authors

H. Xu
xuhg@maemail.com.cn
Wannan Medical College, China.
X. Zhang
Wannan Medical College, China.
H. Wang
Wannan Medical College, China.
Y. Zhang
Wannan Medical College, China.
Y. Shi
Washington University Medical School, United States.
X. Zhang
Chinese Academy of Sciences & Shanghai Jiao Tong University School of Medicine, China.
The normal ANK protein has a strong influence on anti-calcification. It is known that TGF-β1 is also able to induce extracellular inorganic pyrophosphate (ePPi) elaboration via the TGF-β1-induced ank gene expression and the mitogen-activated protein kinase (MAPK) signaling acts as a downstream effector of TGF-β1. We hypothesized that the expression of the ank gene is regulated by mechanics through TGF-β1-p38 pathway. In this study, we investigated the mechanism of short-time mechanical tension-induced ank gene expression. We found that the continuous cyclic mechanical tension (CCMT) increased the ank gene expression in the endplate chondrocytes, and there was an increase in the TGF-β1 expression after CCMT stimulation. The ank gene expression significantly increased when treated by TGF-β1 in a dose-dependent manner and decreased when treated by SB431542 (ALK inhibitor) in a dose-dependent manner. Our study results indicate that CCMT-induced ank gene expressions may be regulated by TGF-β1 and p38 MAPK pathway.

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Supporting Agencies

Chinese national natural sciences fund project (30973025, 81272048), Chinese Anhui Province Education Department Key Fund Project (KJ2010A320).
H. Xu, Wannan Medical College
Department of Spine Surgery, Yijishan Hospital
X. Zhang, Wannan Medical College
Department of Spine Surgery, Yijishan Hospital
H. Wang, Wannan Medical College
Department of Spine Surgery, Yijishan Hospital
Y. Zhang, Wannan Medical College
Department of Spine Surgery, Yijishan Hospital
Y. Shi, Washington University Medical School
Department of Medicine
X. Zhang, Chinese Academy of Sciences & Shanghai Jiao Tong University School of Medicine
Key Laboratory of Stem Cell Biology

How to Cite



1.
Xu H, Zhang X, Wang H, Zhang Y, Shi Y, Zhang X. Continuous cyclic mechanical tension increases ank expression in endplate chondrocytes through the TGF-β1 and p38 pathway. Eur J Histochem [Internet]. 2013 Sep. 25 [cited 2025 Nov. 11];57(3):e28. Available from: https://www.ejh.it/ejh/article/view/ejh.2013.e28

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