STAT3 and SOCS3 expression patterns during murine placenta development

  • S. San Martin | sebastian.sanmartin@uv.cl Biomedical Research Centre, School of Medicine, Universidad de Valparaiso, Chile.
  • J. Fitzgerald Placentalabor, Department of Obstetrics, Friedrich-Schiller-University Jena, Germany.
  • M. Weber Placentalabor, Department of Obstetrics, Friedrich-Schiller-University Jena, Germany.
  • M. Párraga Biomedical Research Centre, School of Medicine, Universidad de Valparaiso, Chile.
  • T. Sáez Biomedical Research Centre, School of Medicine, Universidad de Valparaiso, Chile.
  • T.M. Zorn Laboratory of Reproductive and Extracellular Matrix Biology, Institute of Biomedical Sciences, University of Sao Paulo, Brazil.
  • U.R. Markert Placentalabor, Department of Obstetrics, Friedrich-Schiller-University Jena, Germany.

Abstract

Signal transducers and activators of transcription 3 (Stat3) has been identified as an important signal transducer in the invasive phenotype of the trophoblasts cells in in vitro studies. However, the in situ distribution and patterns of expression of this molecule in trophoblast cells during the development of the placenta are still under-elucidated. Mice uteri of gestational ages between 7 and 14 days of pregnancy (dop) were fixed in methacarn and processed with immunoperoxidase techniques for detection of Stat3 and its phosphorylation at serine (p-ser727) residues, as well as the suppressor of cytokine signaling 3 (Socs3) expression. Stat3 was observed at 7 through 9 dop in both the antimesometrial and mesometrial deciduas, while continued immunoreactivity between 10 and 13 dop was seen only in the mesometrial decidua. In the placenta, Stat3 was detected in the cytotrophoblast cells of labyrinth and giant trophoblast cells between 10 and 14 dop. Immunoreactivity for Stat3 was also seen in trophoblast cells surrounding the maternal blood vessels. On days 10 and 11 of pregnancy, p-ser727 was detectable in the mesometrial decidua and in giant trophoblasts, while during 12-14 dop in the spongiotrophoblast region. In addition, Socs3 was immunodetected in maternal and placental tissues, principally in the giant trophoblast cells during the whole period of the study. The present in situ study shows the distribution of Stat3, its serine activation and Socs3 in different maternal and fetal compartments during murine placental development, thus further supporting the idea that they play a role during physiological placentation in mice.

 

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Published
2013-06-28
Section
Original Papers
Supporting Agencies
DIPUV (Universidad de Valparaiso, Valparaiso, Chile, grants no.12/2006, 07/2009 and CI 05/2006). Programa de Investigación Interdisciplinario (PIA) from Comisión Nacional de Investigación en Ciencia y Tecnología (CONICYT) (Anillos ACT-73, Chile) and Progr
Keywords:
Placenta, uterus, Stat3, mouse, decidualization of endometrium
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How to Cite
San Martin, S., Fitzgerald, J., Weber, M., Párraga, M., Sáez, T., Zorn, T., & Markert, U. (2013). STAT3 and SOCS3 expression patterns during murine placenta development. European Journal of Histochemistry, 57(2), e19. https://doi.org/10.4081/ejh.2013.e19