TGF-β1 expression in chromophobe renal cell carcinoma and renal oncocytoma

  • A. Demirovic | alma.demirovic@kbcsm.hr Sestre Milosrdnice University Hospital Centre, Croatia.
  • S. Cesarec Sestre Milosrdnice University Hospital Centre, Croatia.
  • Z. Marusic Sestre Milosrdnice University Hospital Centre,, Croatia.
  • D. Tomas Sestre Milosrdnice University Hospital Centre, Croatia.
  • M. Milosevic Andrija Å tampar School of Public Health, Croatia.
  • T. Hudolin Clinical Hospital Centre Zagreb, Croatia.
  • B. Kruslin Sestre Milosrdnice University Hospital Centre, Croatia.

Abstract

Distinguishing renal oncocytoma (RO) from the eosinophilic variant of chromophobe renal cell carcinoma (ChRCC) under the light microscope is a common diagnostic problem. Our recent research has shown significant difference between the presence of tumor fibrous capsule in ChRCCs and ROs. Transforming growth factor beta 1 (TGF-β1) is a potent cytokine involved in regulating a number of cellular processes. Two main purposes of this research were to investigate whether the TGF-β1 staining could be related to the presence of tumor fibrous capsule and if it could be used in the differential diagnosis between ChRCC and RO. We investigated 34 cases: 16 ChRCCs (8 eosinophilic and 8 classic) and 18 ROs. All available slides of each tumor, routinely stained with hematoxylin and eosin (H&E) were first analyzed to note the presence of tumor fibrous capsule. One paraffin embedded tissue block matching the representative H&E slide was selected for the immunohistochemical analysis. TGF-β1 expression was analyzed semiquantitatively in the tumor tissue, the tumor fibrous capsule, if present and the peritumoral renal parenchyma. Intensity of TGF-β1 expression was weaker in ChRCCs than the one observed in ROs (P<0.05). The type of reaction in ChRCCs was predominantly membranous unlike in ROs, which exhibited a predominantly cytoplasmic reaction (P<0.05). Moreover, none of the ROs showed membranous type of reaction for TGF-β1. In the group of ChRCCs, tumors with capsule had statistically significant higher quantity of TGF-β1 expression in tumor tissue and in peritumoral renal parenchyma compared to the tumors without capsule (P<0.05). Our results showed different types of TGF-β1 expression in ChRCCs and ROs: ChRCCs had predominantly membranous type of reaction, and ROs predominantly cytoplasmic. Furthermore, ChRCCs with capsule had statistically significant higher quantity of TGF-β1 expression in tumor tissue and in peritumoral renal parenchyma compared to the tumors without capsule. Based on these findings we can speculate that it could be possible that TGF-β1 plays a role in the formation of fibrous capsule in ChRCCs.

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Author Biographies

A. Demirovic, Sestre Milosrdnice University Hospital Centre
Department of Pathology
S. Cesarec, Sestre Milosrdnice University Hospital Centre
Department of Pathology
Z. Marusic, Sestre Milosrdnice University Hospital Centre,
Department of Pathology
D. Tomas, Sestre Milosrdnice University Hospital Centre
Department of Pathology
T. Hudolin, Clinical Hospital Centre Zagreb
Department of Urology
B. Kruslin, Sestre Milosrdnice University Hospital Centre
Department of Pathology
Published
2014-01-31
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Section
Brief Reports
Supporting Agencies
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Keywords:
capsule, chromophobe renal cell carcinoma, renal oncocytoma, TGF-β1
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How to Cite
Demirovic, A., Cesarec, S., Marusic, Z., Tomas, D., Milosevic, M., Hudolin, T., & Kruslin, B. (2014). TGF-β1 expression in chromophobe renal cell carcinoma and renal oncocytoma. European Journal of Histochemistry, 58(1). https://doi.org/10.4081/ejh.2014.2265