Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry

  • M. Derenzini | massimo.derenzini@unibo.it University of Bologna, Italy.
  • A. L. Olins University of New England, United States.
  • D. E. Olins University of New England, United States.

Abstract

Ultrastructural studies conducted in situ using conventional transmission electron microscopy have had relatively little impact on defining the structural organization of chromatin. This is due to the fact that in routine transmission electron microscopy, together with the deoxyribonucleoprotein, many different intermingled substances are contrasted, masking the ultrastructure of chromatin. By selective staining of DNA in thin sections, using the Feulgen-like osmium-ammine reaction, these drawbacks have been overcome and worthwhile data have been obtained both on the gross morphology and the ultrastructural-functional organization of chromatin in situ. In the present study these results are reviewed and discussed in light of recent achievements in both interphase nuclear chromatin compartmentalization in interphase nuclei and in the structural organization of chromatin fibers in transcriptionally active and inactive chromatin.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Author Biographies

M. Derenzini, University of Bologna
Department of Experimental, Diagnostic and Specialty Medicine
A. L. Olins, University of New England
Department of Pharmaceutical Sciences, College of Pharmacy
D. E. Olins, University of New England
Department of Pharmaceutical Sciences, College of Pharmacy
Published
2014-01-24
Info
Issue
Section
Reviews - 60th Anniversary EJH
Supporting Agencies
grants for orientated fundamental research from the Italian Ministry of Education, University and Research
Keywords:
chromatin structure in situ, DNA cytochemistry, transmission electron microscopy, chromatin fibers, nucleosomes, osmium-ammine
Statistics
  • Abstract views: 2094

  • PDF: 540
  • Supplementary: 124
  • HTML: 919
How to Cite
Derenzini, M., Olins, A. L., & Olins, D. E. (2014). Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry. European Journal of Histochemistry, 58(1). https://doi.org/10.4081/ejh.2014.2307