Immunohistochemical/histochemical double staining method in the study of the columnar metaplasia of the oesophagus

  • D. Cabibi Department of Sciences for Promotion of Health and Mother and Child care - University of Palermo, Italy.
  • A.G. Giannone | aggianno@alice.it Department of Sciences for Promotion of Health and Mother and Child care - University of Palermo, Italy.
  • C. Mascarella Department of Sciences for Promotion of Health and Mother and Child care - University of Palermo, Italy.
  • C. Guarnotta Department of Sciences for Promotion of Health and Mother and Child care - University of Palermo, Italy.
  • M. Castiglia Department of Surgical, Oncological and Stomatological Disciplines - University of Palermo, Italy.
  • G. Pantuso Department of Surgical, Oncological and Stomatological Disciplines - University of Palermo, Italy.
  • E. Fiorentino Department of Surgical, Oncological and Stomatological Disciplines - University of Palermo, Italy.

Abstract

Intestinal metaplasia in Barrett’s oesophagus (BO) represents an important risk factor for oesophageal adenocarcinoma. Instead, few and controversial data are reported about the progression risk of columnar-lined oesophagus without intestinal metaplasia (CLO), posing an issue about its clinical management. The aim was to evaluate if some immunophenotypic changes were present in CLO independently of the presence of the goblet cells. We studied a series of oesophageal biopsies from patients with endoscopic finding of columnar metaplasia, by performing some immunohistochemical stainings (CK7, p53, AuroraA) combined with histochemistry (Alcian-blue and Alcian/PAS), with the aim of simultaneously assess the histochemical features in cells that shows an aberrant expression of such antigens. We evidenced a cytoplasmic expression of CK7 and a nuclear expression of Aurora A and p53,  both in goblet cells of BO and in non-goblet cells of CLO, some of which showing mild dysplasia. These findings suggest that some immunophenotypic changes are present in CLO and they can precede the appearance of the goblet cells or can be present independently of them, confirming the conception of BO as the condition characterized by any extention of columnar epithelium. This is the first study in which a combined immunohistochemical/histochemical method has been applied to Barrett pathology.

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Published
2014-03-05
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Section
Brief Reports
Keywords:
Barrett’s oesophagus, columnar-lined oesophagus, p53, Aurora A kinase.
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How to Cite
Cabibi, D., Giannone, A., Mascarella, C., Guarnotta, C., Castiglia, M., Pantuso, G., & Fiorentino, E. (2014). Immunohistochemical/histochemical double staining method in the study of the columnar metaplasia of the oesophagus. European Journal of Histochemistry, 58(1). https://doi.org/10.4081/ejh.2014.2326