Immunoreactivities of PPARγ2, leptin and leptin receptor in oviduct of Chinese brown frog during breeding period and pre-hibernation

  • Y. Liu Beijing Forestry University, China.
  • J. Weng Beijing Forestry University, China.
  • S. Huang Beijing Forestry University, China.
  • Y. Shen Beijing Forestry University, China.
  • X. Sheng Beijing Forestry University, China.
  • Y. Han Beijing Forestry University, China.
  • M. Xu Beijing Forestry University, China.
  • Q. Weng | qiangweng@bjfu.edu.cn Beijing Forestry University, China.

Abstract

The Chinese brown frog (Rana dybowskii) is a special amphibian with one unique physiological phenomenon, which is that its oviduct expands prior to hibernation, instead of during the breeding period. In this study, we investigate the localization and expression level of PPARγ2, leptin and leptin receptor proteins in oviduct of Rana dybowskii during  breeding period and pre-hibernation. There were significant variations in oviductal weight and size, with values much lower in the breeding period than in pre-hibernation. PPARγ2 was observed in stromal and epithelial cells in both periods. Leptin was immunolocalized in epithelial cells in both periods, whereas leptin receptor was detected only in stromal cells. Consistently, the protein levels of PPARγ2, leptin and leptin receptor were higher in pre-hibernation as compared to the breeding period. These results suggested that oviduct was the target organ of leptin, which may play an important paracrine role in regulating the oviductal hypertrophy during pre-hibernation.

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Published
2014-09-09
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Original Papers
Supporting Agencies
National Natural Science Foundation of China (NSFC, No. J1103516), Beijing Natural Science Foundation (8142029).
Keywords:
Leptin, leptin receptor, oviduct, PPARγ2, Rana dybowskii.
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How to Cite
Liu, Y., Weng, J., Huang, S., Shen, Y., Sheng, X., Han, Y., Xu, M., & Weng, Q. (2014). Immunoreactivities of PPARγ2, leptin and leptin receptor in oviduct of Chinese brown frog during breeding period and pre-hibernation. European Journal of Histochemistry, 58(3). https://doi.org/10.4081/ejh.2014.2422