Cytoplasmic lattices are not linked to mouse 2-cell embryos developmental arrest

  • Marianna Longo San Matteo foundation for health, hospitalization and care, Research Center for Regenerative Medicine, Italy.
  • Michele Boiani Max Planck Institute for Molecular Biomedicine, Germany. https://orcid.org/0000-0003-2765-2781
  • CarloAlberto Redi University of Pavia, Department of Biology and Biotechnology ‘‘L. Spallanzani’’, Italy. https://orcid.org/0000-0001-5997-7578
  • Manuela Monti | m.monti@smatteo.pv.it San Matteo foundation for health, hospitalization and care, Research Center for Regenerative Medicine, Italy.

Abstract

Cytoplasmic lattices are important regulators of oocyte maturation. They store components of the protein synthesis machinery including ribosomes and, among others, they are involved in the regulation of microtubule dynamics in both mouse and human. Cytoplasmic lattices undergo dramatic reorganizations at crucial stages of oocyte maturation, where they are abundantly present in the cytoplasm of developmentally competent oocytes named SN (Surrounded Nucleolus) while they are rare in the cytoplasm of 2-cell stage-arresting NSN (Not Surrounded Nucleolus) oocytes, suggestive of a requirement of cytoplasmic lattices for development past the 2-cell stage. Here, to elucidate this requirement, 2-cell mouse embryos derived from SN and NSN oocytes were analyzed by transmission electron microscopy. Contrary to what had been proposed hitherto, cytoplasmic lattices are present in 2-cell embryos derived not only from SN, but also from NSN oocytes, irrespective of the embryo production system (intra cytoplasmic sperm injection, parthenogenesis). Hence our conclusion that cytoplasmic lattices do not count among the factor(s) responsible for the embryo arrest at this crucial stage of development.

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Author Biography

Marianna Longo, San Matteo foundation for health, hospitalization and care, Research Center for Regenerative Medicine

MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, UK

Published
2018-09-24
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Issue
Section
Original Papers
Supporting Agencies
Fondazione IRCCS Policlinico San Matteo (grant n. 688-rcr2012-50)
Keywords:
Oocytes;, cytoplasmic lattices, transmission electron microscopy, 2-cell embryo, developmental competence, ICSI, parthenogenesis.
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How to Cite
Longo, M., Boiani, M., Redi, C., & Monti, M. (2018). Cytoplasmic lattices are not linked to mouse 2-cell embryos developmental arrest. European Journal of Histochemistry, 62(4). https://doi.org/10.4081/ejh.2018.2972

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