Localization of CGRP and VEGF mRNAs in the mouse superior cervical ganglion during pre- and postnatal development

  • Kazuyuki Mitsuoka Nippon Dental University Hospital, Division of Orthodontics, Japan.
  • Yoko Miwa Nippon Dental University, Department of Anatomy, Japan.
  • Takeshi Kikutani Nippon Dental University, Department of Clinical Oral Rehabilitation, Japan.
  • Iwao Sato | iwaoa1@tokyo.ndu.ac.jp Nippon Dental University, Department of Anatomy, Japan. https://orcid.org/0000-0002-6727-1329

Abstract

The neuropeptide calcitonin gene-related peptide (CGRP) mediates inflammation and head pain by influencing the functional vascular blood supply. CGRP is a well-characterized mediator of receptor-regulated neurotransmitter release. However, knowledge regarding the role of CGRP during the development of the superior cervical ganglion (SCG) is limited. In the present study, we observed the localization of CGRP and vascular endothelial growth factor (VEGF-A) mRNAs during prenatal development at embryonic day 14.5 (E14.5), E17.5 and postnatal day 1 (P1) using in situ hybridization. The antisense probe for CGRP was detected by in situ hybridization at E14.5, E17.5, and P1, and the highest levels were detected at E17.5. In contrast, the antisense probe for VEGF-A was detected by in situ hybridization in gradually increasing intensity from E14.5 to P1. The differences in the expression of these two markers revealed specific characteristics related to CGRP concentration and release compared to those of VEGF-A during development. The correlation between CGRP and VEGF-A may influence functional stress and the vascular blood supply during prenatal and postnatal development.

Downloads

Download data is not yet available.
Published
2018-11-22
Section
Original Papers
Associate Editor
mouse
Keywords:
Superior cervical ganglion, CGRP, development, ganglion cell
Statistics
Abstract views: 299

PDF: 122
HTML: 6
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Mitsuoka, K., Miwa, Y., Kikutani, T., & Sato, I. (2018). Localization of CGRP and VEGF mRNAs in the mouse superior cervical ganglion during pre- and postnatal development. European Journal of Histochemistry, 62(4). https://doi.org/10.4081/ejh.2018.2976