A method for semi-automated image analysis of HLA class I tumour epithelium expression in rectal cancer

  • Daniëlle Krijgsman Department of Surgery, Leiden University Medical Centre, Leiden, Netherlands.
  • Ronald L.P. van Vlierberghe Department of Surgery, Leiden University Medical Centre, Leiden, Netherlands.
  • Vaios Evangelou Department of Surgery, Leiden University Medical Centre, Leiden, Netherlands.
  • Alexander L. Vahrmeijer Department of Surgery, Leiden University Medical Centre, Leiden, Netherlands.
  • Cornelis J.H. van de Velde Department of Surgery, Leiden University Medical Centre, Leiden, Netherlands.
  • Cornelis F.M. Sier Department of Surgery, Leiden University Medical Centre, Leiden, Netherlands.
  • Peter J.K. Kuppen | P.J.K.Kuppen@lumc.nl Department of Surgery, Leiden University Medical Centre, Leiden, Netherlands.

Abstract

Biomarkers may hold the key towards development and improvement of personalized cancer treatment. For instance, tumour expression of immune system-related proteins may reveal the tumour immune status and, accordingly, determine choice for type of immunotherapy. Therefore, objective evaluation of tumour biomarker expression is needed but often challenging. For instance, human leukocyte antigen (HLA) class I tumour epithelium expression is cumbersome to quantify by eye due to its presence on both tumour epithelial cells and tumour stromal cells, as well as tumour-infiltrating immune cells. In this study, we solved this problem by setting up an immunohistochemical (IHC) double staining using a tissue microarray (TMA) of rectal tumours wherein HLA class I expression was coloured with a blue chromogen, whereas non-epithelial tissue was visualized with a brown chromogen. We subsequently developed a semi-automated image analysis method that identified tumour epithelium as well as the percentage of HLA class I-positive tumour epithelium. Using this technique, we compared HCA2/HC10 and EMR8-5 antibodies for the assessment of HLA class I tumour expression and concluded that EMR8-5 is the superior antibody for this purpose. This IHC double staining can in principle be used for scoring of any biomarker expressed by tumour epithelium.

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Published
2019-05-20
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Original Papers
Keywords:
Automated image analysis, MHC class I, tumour epithelium, digital pathology, biomarkers, cross-reactivity
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How to Cite
Krijgsman, D., van Vlierberghe, R. L., Evangelou, V., Vahrmeijer, A. L., van de Velde, C. J., Sier, C. F., & Kuppen, P. J. (2019). A method for semi-automated image analysis of HLA class I tumour epithelium expression in rectal cancer. European Journal of Histochemistry, 63(2). https://doi.org/10.4081/ejh.2019.3028