Role of Zip1 in the regulation of NPY expression by MLT to promote fracture healing in rats

  • Junjun Shang Graduate Training Base of Jinzhou Medical University, Air Force Hospital of the Northern Theater of Chinese People's Liberation Army (PLA), Shenyang, Liaoning, China.
  • Bin Ma Division of Spine Surgery, Department of Orthopaedics, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
  • Guiling Zhu Department of Emergency Medicine, Zoucheng People’s Hospital, Jining, China.
  • Penghong Dong Department of Critical Medicine, 242 Hospital, Shenyang, Liaoning, China.
  • Chan Wang Shenyang Children’s Hospital, Shenyang, Liaoning, China.
  • Xiaochuan Gu Department of Orthopedics, Changhai Hospital, Navy Medical University, Shanghai, China.
  • Ying Zi | kwnxwp@163.com Department of Emergency Medicine, Graduate Training Base of Jinzhou Medical University, Air Force Hospital of the Northern Theater of Chinese People's Liberation Army (PLA), Shenyang, Liaoning, China.

Abstract

Our previous study documented that melatonin (MLT) induced the osteogenic differentiation of mesenchymal stem cells (MSCs) and promoted the healing of femoral fractures in rats via the neuropeptide Y (NPY)/neuropeptide Y1 receptor (NPY1R) signaling pathway. MLT treatment upregulated the expression of the zinc uptake transporter zinc transporter 1 (Zip1) in nerve cells. Prior research demonstrated that oral zinc upregulated NPY expression. MSCs were isolated from rat bone marrow and identified using flow cytometry in our study. The results showed that MLT treatment upregulated NPY and NPY1R levels in MSCs with osteogenic differentiation, which was accompanied by upregulated Zip1 expression. However, the MLT-induced osteogenic differentiation of MSCs was reversed after interference of Zip1 expression. It was confirmed by the decreased alkaline phosphatase (ALP) level; downregulated activities of type I collagen α1 chain (COL1A1), osteocalcin (OCN), runt-related transcription factor 2 (Runx2) and ALP; and reduced mineralized nodule formation. MLT promoted fracture healing in rats with femoral fracture, which was accompanied by increased expression of NPY and NPY1R and significantly increased expression of Zip1. In contrast, the silencing of Zip1 expression reversed MLT-mediated fracture healing. In summary, Zip1 participated in the regulation of the NPY/NPY1R signaling pathway via MLT to promote the osteogenic differentiation of MSCs and fracture healing.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.
Published
2020-12-02
Info
Issue
Section
Special Issue - Stem cells and regenerative medicine
Keywords:
Zip1 protein, lentivirus, melatonin, neuropeptide Y, neuropeptide Y receptor Y1, mesenchymal stem cells, fracture healing
Statistics
  • Abstract views: 164

  • PDF: 90
  • HTML: 0
How to Cite
Shang, J., Ma, B., Zhu, G., Dong, P., Wang, C., Gu, X., & Zi, Y. (2020). Role of Zip1 in the regulation of NPY expression by MLT to promote fracture healing in rats. European Journal of Histochemistry, 64(s2). https://doi.org/10.4081/ejh.2020.3183