Parkia pendula lectin as histochemistry marker for meningothelial tumour

  • EIC Beltrão | support@pagepress.org
  • PL Medeiros
  • OG Rodrigues
  • J Figueredo-Silva
  • MM Valença
  • LCBB Coelho
  • LB Carvalho

Abstract

Lectins have been intensively used in histochemical techniques for cell surface characterization. These proteins are involved in several biological processes and their use as histochemical markers have been evaluated since they can indicate differences in cell surfaces. Parkia pendula lectin (PpeL) was evaluated as histochemical marker for meningothelial meningioma biopsies. Tissue slices were incubated with PpeL conjugated to horseradish peroxidase (PpeL-HRP) and Concanavalin A-HRP (ConA-HPR) and the binding visualized with diaminobenzidine and hydrogen peroxide. The lectin-tissue binding was inhibited with D-glucose. PpeL showed to be a useful tool for the characterization of meningothelial tumour and clinico-pathological diagnosis.

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Published
2009-06-26
Section
Original Papers
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How to Cite
BeltrãoE., Medeiros, P., Rodrigues, O., Figueredo-Silva, J., Valença, M., Coelho, L., & Carvalho, L. (2009). Parkia pendula lectin as histochemistry marker for meningothelial tumour. European Journal of Histochemistry, 47(2), 139-142. https://doi.org/10.4081/819