Monitoring stress in fish by applying image analysis to their skin mucous cells

  • I. N. Vatsos | ivatsos@vet.auth.gr Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Ichthyology Lab, Greece.
  • Y. Kotzamanis Institute of Aquaculture, Hellenic Centre for Marine Research, Athens, Greece.
  • M. Henry Institute of Aquaculture, Hellenic Centre for Marine Research, Athens, Greece.
  • P. Angelidis Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Ichthyology Lab, Greece.
  • M. Alexis Institute of Aquaculture, Hellenic Centre for Marine Research, Athens, .

Abstract

Several authors have previously demonstrated that the number of the skin mucous cells of fish is affected by many stressors. In the present study, two experiments were conducted in order to examine the effects of two common environmental conditions on the morphology of skin of sea bass and particularly on the number and diameter of skin mucous cells. In the first experiment, two groups of sea bass (mean weight 155.6±10.3 g SD) were maintained in two different concentrations of nitrate, 100 and 700 ppm respectively, for 48 h, while a third group was used as control. In the second experiment, sea bass (initial mean weight 78.9±3.1 g SD) were divided into four groups and each group was maintained in a different level of oxygen for 9 weeks. The oxygen concentration in each group was: 3.6±0.2 ppm, 4.7±0.2 ppm, 6.2±0.2 ppm and 8.2±0.2 ppm. In both experiments the effects of the two environmental factors on the morphology of the fish skin were examined histologically and a software containing a visual basic script macro, allowing quantification of the skin mucous cells, was used to analyze the skin tissue sections. Concerning the overall morphology of the skin and the diameter of the skin mucous cells, no differences were noted in both experiments (P>0.05). It was demonstrated however, that fish maintained in the lowest oxygen level and fish maintained in the highest concentration of nitrate exhibited significantly increased number of mucous cells per skin area (mm2). There is evidence that the enumeration of the skin mucous cells of fish can be used to monitor stress in fish.

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Author Biographies

I. N. Vatsos, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Ichthyology Lab
Lecturer,
Department of Animal Production, Ichthyology
Ecology and Protection of Environment
Y. Kotzamanis, Institute of Aquaculture, Hellenic Centre for Marine Research, Athens

Research Scientist, Grade C

Institute of Aquaculture

M. Henry, Institute of Aquaculture, Hellenic Centre for Marine Research, Athens

Research Scientist

Institute of Aquaculture

P. Angelidis, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Ichthyology Lab

Assistant professor

Department of Animal Production, Ichthyology
Ecology and Protection of Environment

M. Alexis, Institute of Aquaculture, Hellenic Centre for Marine Research, Athens

Research Scientist, grade A

Institute of Aquaculture

Published
2010-05-10
Section
Original Papers
Keywords:
fish, image analysis, mucous cells, nitrate, oxygen, stress.
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How to Cite
Vatsos, I. N., Kotzamanis, Y., Henry, M., Angelidis, P., & Alexis, M. (2010). Monitoring stress in fish by applying image analysis to their skin mucous cells. European Journal of Histochemistry, 54(2), e22. https://doi.org/10.4081/ejh.2010.e22