The expression of Sirtuins 1 and 4 in peripheral blood leukocytes from patients with type 2 diabetes

  • R. Song Shantou University Medical College, China.
  • W. Xu | xuwcan@163.com The First Affiliated Hospital of Shantou University Medical College, China.
  • Y. Chen The First Affiliated Hospital of Shantou University Medical College, China.
  • Z. Li The First Affiliated Hospital of Shantou University Medical College, China.
  • Y. Zeng Shantou University Medical College, China.
  • Y. Fu Shantou University Medical College, China.

Abstract

This study was designed to investigate the relationship between SIRTs 1 and 4 in peripheral blood leukocytes (PBLs) and human type 2 diabetes mellitus (T2DM). SIRTs 1 and 4 have been confirmed to be associated with homeostasis of glucose/lipid metabolism, but their roles in T2DM are still poorly understood. Peripheral blood and biochemical data were collected from 52 healthy individuals (normal control group, NC group) and 113 cases of T2DM patients. Immunocytochemical staining was used to detect SIRT1 and SIRT4 and reverse transcription polymerase chain reaction (RT-PCR) was used to detect SIRT1 or SIRT4 mRNA levels in PBLs. Immunocytochemical staining showed that SIRT1 is expressed in both nucleus and cytoplasm, and SIRT4 in the cytoplasm of granulocytes and monocytes. No SIRT1 or SIRT4 was found in lymphocytes. RT-PCR showed that SIRT1 and SIRT4 mRNA levels in T2DM group were lower than those in NC group (P<0.01). Correlation analysis showed that there is a negative correlation between SIRTs 1 and 4 and fasting plasma glucose (FPG) (P<0.05) (r= -0.161 and -0.156), a positive correlation between SIRT4 mRNA levels and triglyceride (TG)/lipoprotein a (LPa) levels (P<0.05), and a negative correlation between SIRT4 mRNA levels and high density lipoprotein cholesterol (HDL) (P<0.05). SIRTs 1 and 4 may have a role in the pathogenesis of T2DM and their expression in granulocytes and monocytes may indirectly reflect the homeostasis of glucose/lipid metabolism in T2DM.

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Published
2011-03-21
Section
Original Papers
Supporting Agencies
Laboratory of Cell Senescence, Shantou University Medical College
Keywords:
SIRT1, SIRT4, type 2 diabetes, peripheral blood leukocytes, pancreatic islet ?-cell
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How to Cite
Song, R., Xu, W., Chen, Y., Li, Z., Zeng, Y., & Fu, Y. (2011). The expression of Sirtuins 1 and 4 in peripheral blood leukocytes from patients with type 2 diabetes. European Journal of Histochemistry, 55(1), e10. https://doi.org/10.4081/ejh.2011.e10