Cellular and tissue expression of DAPIT, a phylogenetically conserved peptide

Abstract

DAPIT (Diabetes Associated Protein in Insulin-sensitive Tissues) is a small, phylogenetically conserved, 58 amino acid peptide that was previously shown to be down-regulated at mRNA level in insulin-sensitive tissues of type 1 diabetes rats. In this study we characterize a custom made antibody against DAPIT and confirm the mitochondrial presence of DAPIT on cellular level. We also show that DAPIT is localized in lysosomes of HUVEC and HEK 293T cells. In addition, we describe the histological expression of DAPIT in several tissues of rat and man and show that it is highly expressed especially in cells with high aerobic metabolism and epithelial cells related to active transport of nutrients and ions. We propose that DAPIT, in addition to indicated subunit of mitochondrial F-ATPase, is also a subunit of lysosomal V-ATPase suggesting that it is a common component in different proton pumps.

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Author Biographies

H. Kontro

Institute of Biomedical Technology, and Pediatric Research Center

Graduate Student

J.J. Hulmi

Department of Biology oand Physical Activity

Post Doctoral Fellow

P. Rahkila

Department of Health Sciences

Project Researcher

H. Kainulainen

Department of Biology and Physical Activity

Professor of exercise physiology

Published
2012-05-22
Section
Original Papers
Supporting Agencies
The Competitive Research Funding of the Pirkanmaa Hospital District, The Diabetes Research Foundation, The Finnish Cultural Foundation, Pirkanmaa Regional fund, The Academy of Finland
Keywords:
DAPIT, mitochondrion, V-ATPase, type 1 diabetes
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How to Cite
Kontro, H., Hulmi, J., Rahkila, P., & Kainulainen, H. (2012). Cellular and tissue expression of DAPIT, a phylogenetically conserved peptide. European Journal of Histochemistry, 56(2), e18. https://doi.org/10.4081/ejh.2012.18