Cellular and tissue expression of DAPIT, a phylogenetically conserved peptide


DAPIT (Diabetes Associated Protein in Insulin-sensitive Tissues) is a small, phylogenetically conserved, 58 amino acid peptide that was previously shown to be down-regulated at mRNA level in insulin-sensitive tissues of type 1 diabetes rats. In this study we characterize a custom made antibody against DAPIT and confirm the mitochondrial presence of DAPIT on cellular level. We also show that DAPIT is localized in lysosomes of HUVEC and HEK 293T cells. In addition, we describe the histological expression of DAPIT in several tissues of rat and man and show that it is highly expressed especially in cells with high aerobic metabolism and epithelial cells related to active transport of nutrients and ions. We propose that DAPIT, in addition to indicated subunit of mitochondrial F-ATPase, is also a subunit of lysosomal V-ATPase suggesting that it is a common component in different proton pumps.


Download data is not yet available.

Author Biographies

H. Kontro

Institute of Biomedical Technology, and Pediatric Research Center

Graduate Student

J.J. Hulmi

Department of Biology oand Physical Activity

Post Doctoral Fellow

P. Rahkila

Department of Health Sciences

Project Researcher

H. Kainulainen

Department of Biology and Physical Activity

Professor of exercise physiology

Original Papers
Supporting Agencies
The Competitive Research Funding of the Pirkanmaa Hospital District, The Diabetes Research Foundation, The Finnish Cultural Foundation, Pirkanmaa Regional fund, The Academy of Finland
DAPIT, mitochondrion, V-ATPase, type 1 diabetes
Abstract views: 541

PDF: 271
HTML: 4748
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Kontro, H., Hulmi, J., Rahkila, P., & Kainulainen, H. (2012). Cellular and tissue expression of DAPIT, a phylogenetically conserved peptide. European Journal of Histochemistry, 56(2), e18. https://doi.org/10.4081/ejh.2012.18