Ultrastructure of human mature oocytes after vitrification

Submitted: 17 June 2012
Accepted: 2 July 2012
Published: 10 August 2012
Abstract Views: 1147
PDF: 1493
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Authors

Since the introduction of human assisted reproduction, oocyte cryopreservation has been regarded as an attractive option to capitalize the reproductive potential of surplus oocytes and preserve female fertility. However, for two decades the endeavor to store oocytes has been limited by the not yet optimized methodologies, with the consequence of poor clinical outcome or of uncertain reproducibility. Vitrification has been developed as the promising technology of cryopreservation even if slow freezing remains a suitable choice. Nevertheless, the insufficiency of clinical and correlated multidisciplinary data is still stirring controversy on the impact of this technique on oocyte integrity. Morphological studies may actually provide a great insight in this debate. Phase contrast microscopy and other light microscopy techniques, including cytochemistry, provided substantial morphofunctional data on cryopreserved oocyte, but are unable to unraveling fine structural changes. The ultrastructural damage is one of the most adverse events associated with cryopreservation, as an effect of cryo-protectant toxicity, ice crystal formation and osmotic stress. Surprisingly, transmission electron microscopy has attracted only limited attention in the field of cryopreservation. In this review, the subcellular structure of human mature oocytes following vitrification is discussed at the light of most relevant ultrastructural studies.

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Supporting Agencies

University of Rome La Sapienza, University of L'Aquila

How to Cite

Khalili, M., Maione, M., Palmerini, M., Bianchi, S., Macchiarelli, G., & Nottola, S. (2012). Ultrastructure of human mature oocytes after vitrification. European Journal of Histochemistry, 56(3), e38. https://doi.org/10.4081/ejh.2012.e38