https://doi.org/10.4081/ejh.2013.e23

In vitro reparative dentin: a biochemical and morphological study

Vol. 57 No. 3 (2013)
Submitted: 24 May 2013
Accepted: 27 June 2013
Published: 6 August 2013
dental tissue, in vitro differentiation, DMP1, DSP, TYPE I COLLAGEN .
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Authors

G. Teti
University of Bologna, Italy.
V. Salvatore
University of Bologna, Italy.
A. Ruggeri
University of Bologna, Italy.
L. Manzoli
University of Bologna, Italy.
M. Gesi
University of Pisa, Italy.
G. Orsini
Polytechnic University of Marche, Italy.
M. Falconi
mirella.falconi@unibo.it
University of Bologna, Italy.

In this study, starting from human dental pulp cells cultured in vitro, we simulated reparative dentinogenesis using a medium supplemented with different odontogenic inductors. The differentiation of dental pulp cells in odontoblast-like cells was evaluated by means of staining, and ultramorphological, biochemical and biomolecular methods. Alizarin red staining showed mineral deposition while transmission electron microscopy revealed a  synthesis of extracellular matrix fibers during the differentiation process. Biochemical assays demonstrated that the differentiated phenotype expressed odontoblast markers, such as Dentin Matrix Protein 1 (DMP1) and Dentin Sialoprotein (DSP), as well as type I collagen. Quantitative data regarding the mRNA expression of DMP1, DSP and type I  collagen were obtained by Real Time PCR. Immunofluorescence data demonstrated the various localizations of DSP and DMP1 during odontoblast differentiation. Based on our results, we obtained odontoblast-like cells which simulated the reparative dentin processes in order to better investigate the mechanism of odontoblast differentiation, and dentin extracellular matrix deposition and mineralization.

 

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Supporting Agencies

supported by the Italian Ministry of Research and Technology (MURST) with an FIRB grant (RBAP10 MLK7_005) and a PRIN 2009 grant

How to Cite

Teti, G., Salvatore, V., Ruggeri, A., Manzoli, L., Gesi, M., Orsini, G., & Falconi, M. (2013). In vitro reparative dentin: a biochemical and morphological study. European Journal of Histochemistry, 57(3), e23. https://doi.org/10.4081/ejh.2013.e23

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