In vitro reparative dentin: a biochemical and morphological study

Submitted: 24 May 2013
Accepted: 27 June 2013
Published: 6 August 2013
Abstract Views: 2165
PDF: 572
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In this study, starting from human dental pulp cells cultured in vitro, we simulated reparative dentinogenesis using a medium supplemented with different odontogenic inductors. The differentiation of dental pulp cells in odontoblast-like cells was evaluated by means of staining, and ultramorphological, biochemical and biomolecular methods. Alizarin red staining showed mineral deposition while transmission electron microscopy revealed a  synthesis of extracellular matrix fibers during the differentiation process. Biochemical assays demonstrated that the differentiated phenotype expressed odontoblast markers, such as Dentin Matrix Protein 1 (DMP1) and Dentin Sialoprotein (DSP), as well as type I collagen. Quantitative data regarding the mRNA expression of DMP1, DSP and type I  collagen were obtained by Real Time PCR. Immunofluorescence data demonstrated the various localizations of DSP and DMP1 during odontoblast differentiation. Based on our results, we obtained odontoblast-like cells which simulated the reparative dentin processes in order to better investigate the mechanism of odontoblast differentiation, and dentin extracellular matrix deposition and mineralization.




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Supporting Agencies

supported by the Italian Ministry of Research and Technology (MURST) with an FIRB grant (RBAP10 MLK7_005) and a PRIN 2009 grant

How to Cite

Teti, G., Salvatore, V., Ruggeri, A., Manzoli, L., Gesi, M., Orsini, G., & Falconi, M. (2013). In vitro reparative dentin: a biochemical and morphological study. European Journal of Histochemistry, 57(3), e23.