In vitro reparative dentin: a biochemical and morphological study

Submitted: 24 May 2013
Accepted: 27 June 2013
Published: 6 August 2013
Abstract Views: 2165
PDF: 572
HTML: 927
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

In this study, starting from human dental pulp cells cultured in vitro, we simulated reparative dentinogenesis using a medium supplemented with different odontogenic inductors. The differentiation of dental pulp cells in odontoblast-like cells was evaluated by means of staining, and ultramorphological, biochemical and biomolecular methods. Alizarin red staining showed mineral deposition while transmission electron microscopy revealed a  synthesis of extracellular matrix fibers during the differentiation process. Biochemical assays demonstrated that the differentiated phenotype expressed odontoblast markers, such as Dentin Matrix Protein 1 (DMP1) and Dentin Sialoprotein (DSP), as well as type I collagen. Quantitative data regarding the mRNA expression of DMP1, DSP and type I  collagen were obtained by Real Time PCR. Immunofluorescence data demonstrated the various localizations of DSP and DMP1 during odontoblast differentiation. Based on our results, we obtained odontoblast-like cells which simulated the reparative dentin processes in order to better investigate the mechanism of odontoblast differentiation, and dentin extracellular matrix deposition and mineralization.

 

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Supporting Agencies

supported by the Italian Ministry of Research and Technology (MURST) with an FIRB grant (RBAP10 MLK7_005) and a PRIN 2009 grant

How to Cite

Teti, G., Salvatore, V., Ruggeri, A., Manzoli, L., Gesi, M., Orsini, G., & Falconi, M. (2013). In vitro reparative dentin: a biochemical and morphological study. European Journal of Histochemistry, 57(3), e23. https://doi.org/10.4081/ejh.2013.e23