miR-126 targeting GOLPH3 inhibits the epithelial-mesenchymal transition of gastric cancer BGC-823 cells and reduces cell invasion

  • Jiancai Ouyang Department of Anesthesiology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • Fuhu Song Department of Anesthesiology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • He Li Department of Anesthesiology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • Rui Yang Department of Anesthesiology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • Haicheng Huang | haicheng_huang@163.com Department of Anesthesiology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China. https://orcid.org/0000-0002-2814-5389

Abstract

The incidence and mortality of gastric cancer have been increasing in recent years. MiR-126 and target genes have been studied in gastric cancer, but their studies with Golgi phosphoprotein 3 (GOLPH3) and related pathways in gastric cancer are rarely reported. In the present study, we aimed to investigate the interaction between the miR-126 and GOLPH3in the progression of gastric cancer. In this study, we revealed the role of miR-126-GOLPH3 axis into regulating the progression of epithelial-mesenchymal transition (EMT) in BGC-823 cell model. Firstly, tumor tissues and adjacent normal tissues were collected from 45 patients with gastric cancer. We found the expression of miR-126 in human tumor tissue was significantly lower than in normal tissue using reverse transcription-polymerase chain reaction (RT-PCR). But the GOLPH3 expression was opposite by the detection of immunohistochemistry, RT-PCR and Western blot. Moreover, we predicted miR-126 targeting GOLPH3 by bioinformatics and confirmed the interaction using luciferase reporter gene system; miR-126 inhibited the proliferation, invasion and EMT progression in BGC-823 cells through overexpressing miR-126; miR-126 negative regulated GOLPH3 expression by overexpressing and interfering miR-126. Finally, we found GOLPH3 could promote proliferation using MTT assay, invasion using Transwell, and EMT progression by inhibiting the expression of E-cadherin, inducing vimentin and N-cadherin in BGC-823 cells. Our results demonstrated that miR-126 inhibits proliferative and invasive ability as well as EMT progression by targeting GOLPH3. This study may provide a new field of vision for targeted treatment of gastric cancer.

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Published
2020-10-28
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Articles
Ethics Approval
Ethical approval was obtained for all experimental procedures by the Ethical Committee of the Southern Medical University, Guangzhou, Guangdong, China
Supporting Agencies
Young Scientists Fund of the National Natural Science Foundation of China
Keywords:
Gastric cancer cells, MicroRNA-126, GOLPH3, Invasion, EMT
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How to Cite
Ouyang, J., Song, F., Li, H., Yang, R., & Huang, H. (2020). miR-126 targeting GOLPH3 inhibits the epithelial-mesenchymal transition of gastric cancer BGC-823 cells and reduces cell invasion . European Journal of Histochemistry, 64(4). https://doi.org/10.4081/ejh.2020.3168