https://doi.org/10.4081/ejh.2022.3541

Chemotherapy-induced nephrotoxicity was improved by crocin in mouse model

Vol. 66 No. 4 (2022)
Submitted: 30 August 2022
Accepted: 26 September 2022
Published: 3 October 2022
Crocin, cisplatin, nephrotoxicity, p38 MAPK, caspase-3
Abstract Views: 635
PDF: 423
HTML: 19
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Qichao Yin
https://orcid.org/0000-0002-4358-1971
Department of Hematology, the Affiliated Hospital of Qinghai University, Xining, China.
Hua Xiong
330963014@qq.com
https://orcid.org/0000-0002-5631-6966
Department of Hematology, the Affiliated Hospital of Qinghai University, Xining, China.

Cisplatin (CDDP) has been widely used in cancer therapy, but it has been linked to side effects such as nephrotoxicity. Crocin is a carotenoid found in crocus and gardenia flowers that has been shown to have anti-oxidant properties, inhibit tumor growth, and provide neuroprotection. The purpose of this study was to investigate the protective effect of crocin against CDDP-induced nephrotoxicity in a mouse model. Kunming mice were administered orally with crocin for 7 days at the dose of 6.25 mg/kg and 12.5 mg/kg per body weight daily and were injected with CDDP via intraperitoneal route at the dose of 10 mg/kg per body weight. Using commercial kits, the oxidative stress markers glutathione, malondialdehyde, catalase, glutathione peroxidase, and superoxide dismutase were measured in the kidneys of mice. Immunohistochemistry was used to assess the levels of p53, cleaved caspase-3, and phospho-p38 mitogen-activated protein kinase in the kidneys. Crocin significantly reduced CDDP-induced changes in serum creatinine and blood urea nitrogen levels, according to the findings. Crocin reduced malondialdehyde levels and increased glutathione, glutathione peroxidase, catalase, and superoxide dismutase levels in CDDP-induced lipid peroxidation. Crocin also significantly inhibited p38 mitogen-activated protein kinase activation, p53 expression, and caspase-3 cleavage. In conclusion, crocin protects against CDDP-induced oxidative stress and nephrotoxicity by attenuating the activation of p38 mitogen-activated protein kinase and caspase-3 cleavage.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Crossref
Scopus
Google Scholar
Europe PMC
Manohar S, Leung N. Cisplatin nephrotoxicity: a review of the literature. J Nephrol 2018;31:15-25. DOI: https://doi.org/10.1007/s40620-017-0392-z
Wang Z, Wu R, Nie Q, Bouchonville KJ, Diasio RB, Offer SM. Chromatin assembly factor 1 suppresses epigenetic reprogramming toward adaptive drug resistance. J Natl Cancer Cent 2021;1:15-22. DOI: https://doi.org/10.1016/j.jncc.2020.12.003
Yoon S P, Kim J. Exogenous spermidine ameliorates tubular necrosis during cisplatin nephrotoxicity. Anat Cell Biol 2018;51:189-99. DOI: https://doi.org/10.5115/acb.2018.51.3.189
Ahmed OM, Hassan MA, Saleh AS. Combinatory effect of hesperetin and mesenchymal stem cells on the deteriorated lipid profile, heart and kidney functions and antioxidant activity in STZ-induced diabetic rats. Biocell 2020;44:27-9. DOI: https://doi.org/10.32604/biocell.2020.08040
Neamatallah T, El-Shitany N, Abbas A, Eid BG, Harakeh S, Ali S, et al. Nano ellagic acid counteracts cisplatin-induced upregulation in OAT1 and OAT3: a possible nephroprotection mechanism. Molecules 2020;25:3031. DOI: https://doi.org/10.3390/molecules25133031
Huang H, Miao H, Wang J, Wu D, Lei Q, Wang S, et al. Advances on anticancer new drugs in China and the USA in 2020: from ongoing trial to drug approval. J Natl Cancer Cent 2021;1:147-52. DOI: https://doi.org/10.1016/j.jncc.2021.08.002
Kim JY, Jo J, Leem J, Park KK. Inhibition of p300 by garcinol protects against cisplatin-induced acute kidney injury through suppression of oxidative stress, inflammation, and tubular cell death in mice. Antioxidants 2020;9:1271. DOI: https://doi.org/10.3390/antiox9121271
Owumi SE, Dim UJ. Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos. Toxicol Rep 2019;6:202-9. DOI: https://doi.org/10.1016/j.toxrep.2019.02.007
Heitmar R, Brown J, Kyrou I. Saffron (Crocus sativus L.) in ocular diseases: a narrative review of the existing evidence from clinical studies. Nutrients 2019;11:649. DOI: https://doi.org/10.3390/nu11030649
Lambrianidou A, Koutsougianni F, Papapostolou I, Dimas K. Recent advances on the anticancer properties of Saffron (Crocus sativus L.) and its major constituents. Molecules 2020;26:86. DOI: https://doi.org/10.3390/molecules26010086
Sheng K, Wei S, Mei J, Xie J. Chilling injury, physicochemical properties, and antioxidant enzyme activities of red pitahaya (Hylocereus polyrhizus) fruits under cold storage stress. Phyton-Int J Exp Botany 2021;90:291-305. DOI: https://doi.org/10.32604/phyton.2020.012985
Perše M, Večerić-Haler Ž. Cisplatin-induced rodent model of kidney injury: characteristics and challenges. Biomed Res Int 2018;2018:1462802. DOI: https://doi.org/10.1155/2018/1462802
Sohail N, Hira K, Tariq A, Sultana V, Ehteshamul-Haque S. Marine macro-algae attenuates nephrotoxicity and hepatotoxicity induced by cisplatin and acetaminophen in rats. Environ Sci Pollut Res Int 2019;26:25301-11. DOI: https://doi.org/10.1007/s11356-019-05704-y
Volarevic V, Djokovic B, Jankovic MG, Harrell CR, Fellabaum C, Djonov V, et al. Molecular mechanisms of cisplatin-induced nephrotoxicity: a balance on the knife edge between renoprotection and tumor toxicity. J Biomed Sci 2019;26:25. DOI: https://doi.org/10.1186/s12929-019-0518-9
Li W, Yang Y, Li Y, Zhao Y, Jiang H. Sirt5 attenuates cisplatin-induced acute kidney injury through regulation of Nrf2/HO-1 and Bcl-2. Biomed Res Int 2019;2019:4745132. DOI: https://doi.org/10.1155/2019/4745132
Santos NA, Catão CS, Martins NM, Curti C, Bianchi ML, Santos AC. Cisplatin-induced nephrotoxicity is associated with oxidative stress, redox state unbalance, impairment of energetic metabolism and apoptosis in rat kidney mitochondria. Arch Toxicol 2007;81:495-504. DOI: https://doi.org/10.1007/s00204-006-0173-2
Qi Z, Li Z, Li W, Liu Y, Wang C, Lin H, et al. Pseudoginsengenin DQ exhibits therapeutic effects in cisplatin-induced acute kidney injury via Sirt1/NF-κB and caspase signaling pathway without compromising its antitumor activity in mice. Molecules 2018;23:3038. DOI: https://doi.org/10.3390/molecules23113038
Un H, Ugan RA, Gurbuz MA, Bayir Y, Kahramanlar A, Kaya G, et al. Phloretin and phloridzin guard against cisplatin-induced nephrotoxicity in mice through inhibiting oxidative stress and inflammation. Life Sci 2021;266:118869. DOI: https://doi.org/10.1016/j.lfs.2020.118869
Ahmed OM, Galaly SR, Raslan M, Mostafa MMA. Thyme oil and thymol abrogate doxorubicin-induced nephrotoxicity and cardiotoxicity in Wistar rats via repression of oxidative stress and enhancement of antioxidant defense mechanisms. Biocell 2020;44:41-53. DOI: https://doi.org/10.32604/biocell.2020.08157
Sheth S, Mukherjea D, Rybak LP, Ramkumar V. Mechanisms of cisplatin-induced ototoxicity and otoprotection. Front Cell Neurosci 2017;11:338. DOI: https://doi.org/10.3389/fncel.2017.00338
Zhou J, Fan Y, Zhong J, Huang Z, Huang T, Lin S, et al. TAK1 mediates excessive autophagy via p38 and ERK in cisplatin-induced acute kidney injury. J Cell Mol Med 2018;22:2908-21. DOI: https://doi.org/10.1111/jcmm.13585
Khan R, Khan AQ, Qamar W, Lateef A, Tahir M, Rehman MU, et al. Chrysin protects against cisplatin-induced colon. toxicity via amelioration of oxidative stress and apoptosis: Probable role of p38MAPK and p53. Toxicol Appl Pharmacol 2012;258:315-29. DOI: https://doi.org/10.1016/j.taap.2011.11.013
Heilig R, Dilucca M, Boucher D, Chen KW, Hancz D, Demarco B, et al. Caspase-1 cleaves Bid to release mitochondrial SMAC and drive secondary necrosis in the absence of GSDMD. Life Sci Alliance 2020;3:e202000735. DOI: https://doi.org/10.26508/lsa.202000735
Huang D, Wang C, Duan Y, Meng Q, Liu Z, Huo X, et al. Targeting Oct2 and P53: Formononetin prevents cisplatin-induced acute kidney injury. Toxicol Appl Pharmacol 2017;326:15-24. DOI: https://doi.org/10.1016/j.taap.2017.04.013

Ethics Approval

All animal experiment protocols listed below have been reviewed and approved by the by Animal Use and Care Committee of Affiliated Hospital of Qinghai University, Xining, China (No. 20200318)

How to Cite

Yin, Q., & Xiong, H. (2022). Chemotherapy-induced nephrotoxicity was improved by crocin in mouse model. European Journal of Histochemistry, 66(4). https://doi.org/10.4081/ejh.2022.3541
Copyright (c) 2022 The Author(s) Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.