https://doi.org/10.4081/ejh.2022.3541

Chemotherapy-induced nephrotoxicity was improved by crocin in mouse model

Vol. 66 No. 4 (2022)
Submitted: 30 August 2022
Accepted: 26 September 2022
Published: 3 October 2022
Crocin, cisplatin, nephrotoxicity, p38 MAPK, caspase-3
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Authors

Qichao Yin
https://orcid.org/0000-0002-4358-1971
Department of Hematology, the Affiliated Hospital of Qinghai University, Xining, China.
Hua Xiong
330963014@qq.com
https://orcid.org/0000-0002-5631-6966
Department of Hematology, the Affiliated Hospital of Qinghai University, Xining, China.

Cisplatin (CDDP) has been widely used in cancer therapy, but it has been linked to side effects such as nephrotoxicity. Crocin is a carotenoid found in crocus and gardenia flowers that has been shown to have anti-oxidant properties, inhibit tumor growth, and provide neuroprotection. The purpose of this study was to investigate the protective effect of crocin against CDDP-induced nephrotoxicity in a mouse model. Kunming mice were administered orally with crocin for 7 days at the dose of 6.25 mg/kg and 12.5 mg/kg per body weight daily and were injected with CDDP via intraperitoneal route at the dose of 10 mg/kg per body weight. Using commercial kits, the oxidative stress markers glutathione, malondialdehyde, catalase, glutathione peroxidase, and superoxide dismutase were measured in the kidneys of mice. Immunohistochemistry was used to assess the levels of p53, cleaved caspase-3, and phospho-p38 mitogen-activated protein kinase in the kidneys. Crocin significantly reduced CDDP-induced changes in serum creatinine and blood urea nitrogen levels, according to the findings. Crocin reduced malondialdehyde levels and increased glutathione, glutathione peroxidase, catalase, and superoxide dismutase levels in CDDP-induced lipid peroxidation. Crocin also significantly inhibited p38 mitogen-activated protein kinase activation, p53 expression, and caspase-3 cleavage. In conclusion, crocin protects against CDDP-induced oxidative stress and nephrotoxicity by attenuating the activation of p38 mitogen-activated protein kinase and caspase-3 cleavage.

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Ethics Approval

All animal experiment protocols listed below have been reviewed and approved by the by Animal Use and Care Committee of Affiliated Hospital of Qinghai University, Xining, China (No. 20200318)

How to Cite

Yin, Q., & Xiong, H. (2022). Chemotherapy-induced nephrotoxicity was improved by crocin in mouse model. European Journal of Histochemistry, 66(4). https://doi.org/10.4081/ejh.2022.3541
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