Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment

  • R.J. BuÅ‚dak | rbuldak@sum.edu.pl Medical University of Silesia, Poland.
  • M. Skonieczna Silesian University of Technology, Poland.
  • Ł. BuÅ‚dak Medical University of Silesia, Poland.
  • N. Matysiak Medical University of Silesia, Poland.
  • Ł. MielaÅ„czyk Medical University of Silesia, Poland.
  • G. Wyrobiec Medical University of Silesia, Poland.
  • M. Kukla Medical University of Silesia, Poland.
  • M. Michalski Medical University of Silesia, Poland.
  • K. Å»wirska-Korczala Medical University of Silesia, Poland.

Abstract

The aim of the study was to assess the expression and subcellular localization of visfatin in HCT-116 colorectal carcinoma cells after cytokinesis failure using Cytochalasin B (CytB) and the mechanism of apoptosis of cells after CytB. We observed translocation of visfatin’s antigen in cytB treated colorectal carcinoma HCT-116 cells from cytosol to nucleus. Statistical and morphometric analysis revealed significantly higher area-related numerical density visfatin-bound nano-golds in the nuclei of cytB-treated HCT-116 cells compared to cytosol. Reverse relation to visfatin subcellular localization was observed in un-treated HCT-116 cells. The total amount of visfatin protein and visfatin mRNA level in HCT-116 cells was also decreased after CytB treatment. Additionally, CytB significantly decreased cell survival, increased levels of G2/M fractions, induced bi-nuclei formation as well as increased reactive oxygen species (ROS) level in HCT-116 cells. CytB treatment showed cytotoxic effect that stem from oxidative stress and is connected with the changes in the cytoplasmic/nuclear amount of visfatin in HCT-116 cells.

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Author Biography

R.J. Bułdak, Medical University of Silesia
Department of Physiology
Published
2014-09-12
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Section
Original Papers
Supporting Agencies
Silesian Medical University in Katowice, Poland. Grant # KNW-2-009/N/3/N, GCONiI - Upper-Silesian Center for Scientific Computation, grant #POIG.02.03.01-24-099/13.
Keywords:
Visfatin, cytochalasin B, immunogold labeling, TEM, adipocytokines.
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How to Cite
Bułdak, R., Skonieczna, M., Bułdak Ł., Matysiak, N., Mielańczyk Ł., Wyrobiec, G., Kukla, M., Michalski, M., & Żwirska-Korczala, K. (2014). Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment. European Journal of Histochemistry, 58(3). https://doi.org/10.4081/ejh.2014.2408