Dipeptidylpeptidase-IV activity and expression reveal decreased damage to the intrahepatic biliary tree in fatty livers submitted to subnormothermic machine-perfusion respect to conventional cold storage

  • E. Tarantola University of Pavia, Italy.
  • V. Bertone University of Pavia, Italy.
  • G. Milanesi University of Pavia, Italy.
  • C. Gruppi University of Pavia, Italy.
  • A. Ferrigno University of Pavia, Italy.
  • M. Vairetti University of Pavia, Italy.
  • S. Barni University of Pavia, Italy.
  • I. Freitas | freitas@unipv.it University of Pavia, Italy.

Abstract

Graft steatosis is a risk factor for poor initial function after liver transplantation. Biliary complications are frequent even after normal liver transplantation. A subnormothermic machine perfusion (MP20) preservation procedure was developed by our group with high potential for reducing injury to hepatocytes and sinusoidal cells of lean and fatty livers respect to conventional cold storage (CS). We report the response of the biliary tree to CS or MP20, in lean and obese Zucker rat liver. Dipeptidylpeptidase-IV (DPP-IV), crucial for the inactivation of incretins and neuropeptides, was used as a marker. Liver morphology and canalicular network of lean livers were similar after CS/reperfusion or MP20/reperfusion. CS preservation of fatty livers induced serious damage to the parenchyma and to the canalicular activity/expression of DPP-IV whereas with MP20 the morphology and canalicular network were similar to those of untreated lean liver. CS and MP20 had similar effects on DPP-IV activity and expression in the upper segments of the intrahepatic biliary tree of fatty livers. DPP-IV expression was significantly increased after MP20 respect to CS or to the controls, both for lean and obese animals. Our data support the superiority of MP20 over CS for preserving fatty livers. Dipeptidylpeptidase-IV activity and expression reveal decreased damage to the intrahepatic biliary tree in fatty livers submitted to subnormothermic machine-perfusion respect to conventional cold storage.

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Author Biographies

E. Tarantola, University of Pavia

Department of Biology and Biotechnology "L.Spallanzani"

Post-doc

V. Bertone, University of Pavia

Department of Biology and Biotechnology "L.Spallanzani"

Researcher

G. Milanesi, University of Pavia

Department of Biology and Biotechnology "L.Spallanzani"

Technician

C. Gruppi, University of Pavia

Department of Molecular Medicine

Post-doc

A. Ferrigno, University of Pavia

Department of Internal Medicine and Therapeutics

Post-doc

M. Vairetti, University of Pavia

Department of Internal Medicine and Therapeutics

Researcher

S. Barni, University of Pavia

Department of Biology and Biotechnology "L.Spallanzani"

Full Professor

I. Freitas, University of Pavia

Department of Biology and Biotechnology "L.Spallanzani"

and

Histochemistry and Cytometry Section, Institute of Molecular Genetics of CNR

Associate Professor

Published
2014-07-17
Section
Original Papers
Keywords:
Dipeptidylpeptidase-IV, fatty liver, bile tract, cold storage, subnormothermic machine perfusion.
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How to Cite
Tarantola, E., Bertone, V., Milanesi, G., Gruppi, C., Ferrigno, A., Vairetti, M., Barni, S., & Freitas, I. (2014). Dipeptidylpeptidase-IV activity and expression reveal decreased damage to the intrahepatic biliary tree in fatty livers submitted to subnormothermic machine-perfusion respect to conventional cold storage. European Journal of Histochemistry, 58(3). https://doi.org/10.4081/ejh.2014.2414