Apigenin induces dermal collagen synthesis via smad2/3 signaling pathway

  • Y. Zhang Shanghai Jiao Tong University, China.
  • J. Wang Shanghai Jiao Tong University, China.
  • X. Cheng Shanghai Jiao Tong University, China.
  • B. Yi Anhui Medical University, China.
  • X. Zhang | xlzhang@sibs.ac.cn Chinese Academy of Sciences & Shanghai Jiao Tong University School of Medicine, China.
  • Q. Li Shanghai Jiao Tong University, China.

Abstract

Decrease in fibroblast-produced collagen has been proven to be the pivotal cause of skin aging, but there is no satisfactory drug which directly increases dermal thickness and collage density. Here we found that a flavonoid natural product, apigenin, could significantly increase collagen synthesis. NIH/3T3 and primary human dermal fibroblasts (HDFs) were incubated with various concentrations of apigenin, with dimethyl sulfoxide (DMSO) serving as the negative control. Real-time reverse-transcription polymerase chain reaction (PCR), Western Blot, and Toluidine blue staining demonstrated that apigenin stimulated type-I and type-III collagen synthesis of fibroblasts on the mRNA and protein levels. Meanwhile, apigenin did not induce expression of alpha smooth muscle actin (α-SMA) in vitro and in vivo, a fibrotic marker in living tissues. Then the production of collagen was confirmed by Masson’s trichrome stain, Picrosirius red stain and immunohistochemistry in mouse models. We also clarified that this compound induced collagen synthesis by activating smad2/3 signaling pathway. Taken together, without obvious influence on fibroblasts’ apoptosis and viability, apigenin could promote the type-I and type-III collagen synthesis of dermal fibroblasts in vitro and in vivo, thus suggesting that apigenin may serve as a potential agent for esthetic and reconstructive skin rejuvenation.

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Author Biographies

Y. Zhang, Shanghai Jiao Tong University
Department of Plastic & Reconstructive Surgery, Shanghai 9th People’s Hospital, School of Medicine,
J. Wang, Shanghai Jiao Tong University
Department of Plastic & Reconstructive Surgery, Shanghai 9th People’s Hospital, School of Medicine
X. Cheng, Shanghai Jiao Tong University
Department of Urology, Renji Hospital, School of Medicine
B. Yi, Anhui Medical University
Clinical College of General Hospital of Beijing Military Region
X. Zhang, Chinese Academy of Sciences & Shanghai Jiao Tong University School of Medicine
The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences (SIBS)
Q. Li, Shanghai Jiao Tong University
Department of Plastic & Reconstructive Surgery, Shanghai 9th People’s Hospital, School of Medicine
Published
2015-04-13
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Section
Original Papers
Supporting Agencies
National Natural Science Foundation of China, National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Technology of China, Chinese Academy of Sciences, Shanghai Municipal Commission of Health and Family Plannin
Keywords:
Apigenin, flavonoid, collagen I/III, fibroblasts, smad2/3.
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How to Cite
Zhang, Y., Wang, J., Cheng, X., Yi, B., Zhang, X., & Li, Q. (2015). Apigenin induces dermal collagen synthesis via smad2/3 signaling pathway. European Journal of Histochemistry, 59(2). https://doi.org/10.4081/ejh.2015.2467