In situ histochemical localisation of alkaloids and acetogenins in the endosperm and embryonic axis of Annona macroprophyllata Donn. Sm. seeds during germination

  • A.E. Brechú-Franco | aliciae@ciencias.unam.mx National Autonomous University of Mexico, Mexico.
  • G. Laguna-Hernández National Autonomous University of Mexico, Mexico.
  • I. De la Cruz-Chacón University of Sciences and Arts of Chiapas, Mexico.
  • A.R. González-Esquinca University of Sciences and Arts of Chiapas, Mexico.

Abstract

Currently, the Annonaceae family is characterised by the production of acetogenins (ACGs), and also by the biosynthesis of alkaloids, primarily benzylisoquinolines derived from tyrosine. The objective of this study was to confirm the presence of alkaloids and acetogenins in the idioblasts of the endosperm and the embryonic axis of A. macroprophyllata seeds in germination. The Dragendorff, Dittmar, Ellram, and Lugol reagents were used to test for alkaloids, and Kedde's reagent was used to determine the presence of acetogenins in fresh sections of the endosperm and embryonic axis of seeds after twelve days of germination. A positive reaction was observed for all the reagents, and the presence of alkaloids and acetogenins was confirmed in the idioblasts of the endosperm and those involved in the differentiation of the embryonic axis of the developing seedling. We concluded that the idioblasts store both metabolites, acetogenins and alkaloids. Beginning at differentiation, the idioblasts of the embryonic axis simultaneously biosynthesise acetogenins and alkaloids that are characteristic of the species during the development of the seedling. The method used here can be applied to histochemically confirm the presence of acetogenins and alkaloids in tissues and structures of the plant in different stages of its life cycle.

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Published
2016-02-01
Section
Brief Reports
Supporting Agencies
UNAM
Keywords:
Annona seeds, acetogenins, Kedde’s reagent, alkaloid’s reagents, histochemistry, idioblasts, embryonic axis.
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How to Cite
Brechú-Franco, A., Laguna-Hernández, G., De la Cruz-Chacón, I., & González-Esquinca, A. (2016). In situ histochemical localisation of alkaloids and acetogenins in the endosperm and embryonic axis of Annona macroprophyllata Donn. Sm. seeds during germination. European Journal of Histochemistry, 60(1). https://doi.org/10.4081/ejh.2016.2568