Lectin-like, oxidized low-density lipoprotein receptor-1-deficient mice show resistance to age-related knee osteoarthritis

  • Kazuhiko Hashimoto | hazzhiko@med.kindai.ac.jp Kindai University Hospital, Japan.
  • Yutaka Oda Kindai University Hospital, Japan.
  • Fumihisa Nakamura Kindai University Hospital, Japan.
  • Ryosuke Kakinoki Kindai University Hospital, Japan.
  • Masao Akagi Kindai University Hospital, Japan.

Abstract

The lectin-like, oxidized low-density lipoprotein (ox-LDL) receptor-1 (LOX-1)/ox-LDL system contributes to atherosclerosis and may be involved in cartilage degeneration. The purpose of this study was to determine whether the LOX-1/ox-LDL system contributes to age-related osteoarthritis (OA) in vivo, using LOX-1 knockout (LOX-1 KO) mice. Knee cartilage from 6, 12, and 18-month old (n = 10/group) C57Bl/6 wild-type (WT) and LOX-1 KO mice was evaluated by determining the Osteoarthritis Research Society International (OARSI) score of Safranin-O stained samples. The prevalence of knee OA in both mouse strains was also investigated. Expression levels of LOX-1, ox-LDL, runt-related transcription factor-2 (Runx2), type-X collagen (COL X), and matrix metalloproteinase-13 (MMP-13) in the articular chondrocytes were analyzed immunohistologically. No significant difference was observed in the mean scores of WT (2.00±0.61) and LOX-1 KO mice (2.00±0.49) at 6 months of age (P=1.00, n=10). At 12 and 18 months of age, the mean scores of LOX-1 KO mice (3.75±0.93 and 5.50±0.78) were significantly lower than those of WT mice (5.25±1.14 and 9.00±1.01; P<0.001 in both cases; n=10). The prevalence of OA in LOX-1 KO mice was lower than that in WT mice at 12 and 18 months of age (40 vs 70%, 70 vs 90%, respectively; n=10). The expression levels of Runx2, COL X, and MMP-13 in articular chondrocytes significantly decreased in LOX-1 KO, mice compared with those in WT mice. The study indicated that the LOX-1/ox-LDL system in chondrocytes plays a role in the pathogenesis of age-related knee OA, which is potentially a target for preventing OA progression.

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Author Biographies

Kazuhiko Hashimoto, Kindai University Hospital
department of orthopedic surgery,MD
Yutaka Oda, Kindai University Hospital
department of orthopedic surgery,MD
Fumihisa Nakamura, Kindai University Hospital
department of orthopedic surgery,MD
Ryosuke Kakinoki, Kindai University Hospital
department of orthopedic surgery, MD,PhD
Masao Akagi, Kindai University Hospital
department of orthopedic surgery, MD,PhD
Published
2017-02-14
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Original Papers
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Keywords:
Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), oxidized low-density lipoprotein (ox-LDL), mouse, knee osteoarthritis, aging.
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How to Cite
Hashimoto, K., Oda, Y., Nakamura, F., Kakinoki, R., & Akagi, M. (2017). Lectin-like, oxidized low-density lipoprotein receptor-1-deficient mice show resistance to age-related knee osteoarthritis. European Journal of Histochemistry, 61(1). https://doi.org/10.4081/ejh.2017.2762

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