Incubation under fluid dynamic conditions markedly improves the structural preservation in vitro of explanted skeletal muscles

  • Flavia Carton University of Verona, Department of Neurological, Biomedical and Movement Sciences, Italy.
  • Laura Calderan University of Verona, Department of Neurological, Biomedical and Movement Sciences, Italy.
  • Manuela Malatesta | manuela.malatesta@univr.it University of Verona, Department of Neurological, Biomedical and Movement Sciences, Italy. http://orcid.org/0000-0001-8196-9232

Abstract

Explanted organs and tissues represent suitable experimental systems mimicking the functional and structural complexity of the living organism, with positive ethical and economic impact on research activities. However, their preservation in culture is generally limited, thus hindering their application as experimental models for biomedical research. In the present study, we investigated the potential of an innovative fluid dynamic culture system to improve the structural preservation in vitro of explanted mouse skeletal muscles (soleus). We used light and transmission electron microscopy to compare the morphological features of muscles maintained either in multiwell plates under conventional conditions or in a bioreactor mimicking the flow of physiological fluids. Our results demonstrate that fluid dynamic conditions markedly slowed the progressive structural deterioration of the muscle tissue occurring during the permanence in the culture medium, prolonging the preservation of some organelles such as mitochondria up to 48 h.

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Published
2017-11-28
Section
Technical Notes
Supporting Agencies
I-Care Foundation
Keywords:
, Skeletal muscle, organ culture, bioreactor, morphology, ultrastructure.
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How to Cite
Carton, F., Calderan, L., & Malatesta, M. (2017). Incubation under fluid dynamic conditions markedly improves the structural preservation in vitro of explanted skeletal muscles. European Journal of Histochemistry, 61(4). https://doi.org/10.4081/ejh.2017.2862