Regenerative potential of the Bichat fat pad determined by the quantification of multilineage differentiating stress enduring cells

  • Giamaica Conti | giamaica.conti@univr.it University of Verona, Department of Neurosciences, Biomedicine and Movement Sciences, Italy.
  • Dario Bertossi University of Verona, Maxillofacial Surgery Division, Italy.
  • Elena Dai Prè University of Verona, Department of Neurosciences, Biomedicine and Movement Sciences, Italy.
  • Chiara Cavallini University of Verona, University Laboratory for Medical Research (LURM), Italy.
  • Maria Teresa Scupoli University of Verona, University Laboratory for Medical Research (LURM), Italy.
  • Giulia Ricciardi University of Verona, Maxillofacial Surgery Division, Italy.
  • Pierpaolo Parnigotto Tissue Engineering and Signaling Foundation (TES), Italy.
  • Yves Saban French Facial Plastic Surgery Society, Department of Otorhinolaryngology, Maxillofacial Surgery, France.
  • Andrea Sbarbati University of Verona, Department of Neurosciences, Biomedicine and Movement Sciences, Italy.
  • Pierfrancesco Nocini University of Verona, Maxillofacial Surgery Division, Italy.

Abstract

Published studies regarding Bichat fat pad focused, quite exclusively, on the implant of this adipose depot for different facial portions reconstruction. The regenerative components of Bichat fat pad were poorly investigated. The present study aimed to describe by an ultrastructural approach the Bichat fat pad, providing novel data at the ultrastructural and cellular level. This data sets improve the knowledge about the usefulness of the Bichat fat pad in regenerative and reconstructive surgery. Bichat fat pads were harvested form eight patients subjected to maxillofacial, dental and aesthetic surgeries. Biopsies were used for the isolation of mesenchymal cell compartment and for ultrastructural analysis. Respectively, Bichat fat pads were either digested and placed in culture for the characterization of mesenchymal stem cells (MSCs) or, were fixed in glutaraldehyde 2% and processed for transmission or scanning electron microscopy. Collected data showed very interesting features regarding the cellular composition of the Bichat fat pad and, in particular, experiments aimed to characterized the MSCs showed the presence of a sub-population of MSCs characterized by the expression of specific markers that allow to classify them as multilineage differentiating stress enduring cells.  This data set allows to collect novel information about regenerative potential of Bichat fat pad that could explain the success of its employment in reconstructive and regenerative medicine.

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Published
2018-10-23
Section
Original Papers
Keywords:
Bichat fat pad, MSCs, MUSE, regenerative potential, TEM, SEM
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How to Cite
Conti, G., Bertossi, D., Dai Prè, E., Cavallini, C., Scupoli, M. T., Ricciardi, G., Parnigotto, P., Saban, Y., Sbarbati, A., & Nocini, P. (2018). Regenerative potential of the Bichat fat pad determined by the quantification of multilineage differentiating stress enduring cells. European Journal of Histochemistry, 62(4). https://doi.org/10.4081/ejh.2018.2900