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LEVERAGING ORGANOID TECHNOLOGIES TO CHARACTERIZE DYNAMIC TUMOR CELL STATES DRIVING TREATMENT RESISTANCE IN PANCREATIC CANCER A. Papargyriou1-6, M. Najajreh1-4, D.P. Cook7, C.H. Maurer1,2, S. Bärthel8, P. Putze8, H.A. Messal9, S.K. Ravichandran1-3, T. Richter10, M. Knolle11, T. Metzler12, A.R. Shastri1,2,3, R. Öllinger13, J. Jasper1,2,3, L. Schmidleitner1,2,3, C.Schneeweis8, H. Ishikawa-Ankerhold14, T. Engleitner13, S. Dobiasch15, I. Heid, M.D. Luecken16, K. Steiger12, G. Kaissis11, J. Van Rheenen9, F.J. Theis10, D. Saur8, R. Rad13, M. Reichert1,2,3,4,5,17,18 | 1Translational Pancreatic Cancer Research Center, Klinik und Poliklinik für Innere Medizin II, Klinikum rechts der Isar, Technical University of Munich, Germany; 2Klinik und Poliklinik für Innere Medizin II, Klinikum rechts der Isar, Technical University of Munich, Germany; 3Center for Functional Protein Assemblies, Technical University of Munich, Garching, Germany; 4Center for Organoid Systems (COS), Technical University of Munich, Garching, Germany; 5Bavarian Cancer Research Center (BZKF), Munich, Germany; 6Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg, Germany; 7University of Ottawa, Faculty of Medicine, Department of Cellular and Molecular Medicine, Ottawa, ON, Canada; 8Chair for Translational Cancer Research and Institute of Experimental Cancer Therapy, Klinikum rechts der Isar, School of Medicine, Technical University of Munich, Germany; 9Division of Molecular Pathology, Oncode Institute, The Netherlands Cancer Institute, Plesmanlaan 121, 1066CX, Amsterdam, The Netherlands. 10Institute of Computational Biology, Helmholtz Center Munich, Neuherberg, Germany; 11Institute of Diagnostic and Interventional Radiology, Klinikum rechts der Isar München, Technical University of Munich, Germany; 12Comparative Experimental Pathology, Institut für Allgemeine Pathologie und Pathologische Anatomie, School of Medicine, Technical University of Munich, Germany; 13Institute of Molecular Oncology and Functional Genomics, School of Medicine, Technical University of Munich, Germany; 14Department of Medicine I, University Hospital of the LudwigMaximilians-University Munich, Germany; 15Department of Radiation Oncology, Technical University of Munich, Germany; 16Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Germany; Member of the German Center for Lung Research (DZL); 17German Cancer Consortium (DKTK), Munich, Germany; 18Munich Institute of Biomedical Engineering (MIBE), Technical University of Munich, Germany
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Adipose-derived stem cells - Methods and protocols
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