Immunocytochemical detection of dentin matrix proteins in primary teeth from patients with dentinogenesis imperfecta associated with osteogenesis imperfecta

  • G. Orsini Polytechnic University of Marche, Italy.
  • A. Majorana University of Brescia, Italy.
  • A. Mazzoni University of Trieste, Italy.
  • A. Putignano Polytechnic University of Marche, Italy.
  • M. Falconi University of Bologna, Italy.
  • A. Polimeni La Sapienza University of Rome, Italy.
  • L. Breschi | lorenzo.breschi@unibo.it University of Bologna, Italy.

Abstract

Dentinogenesis imperfecta determines structural alterations of the collagen structure still not completely elucidated. Immunohistochemical analysis was used to assay Type I and VI collagen, various non-collagenous proteins distribution in human primary teeth from healthy patients or from patients affected by type I dentinogenesis imperfecta (DGI-I) associated with osteogenesis imperfecta (OI). In sound primary teeth, an organized well-known ordered pattern of the type I collagen fibrils was found, whereas atypical and disorganized fibrillar structures were observed in dentin of DGI-I affected patients. Expression of type I collagen was observed in both normal and affected primary teeth, although normal dentin stained more uniformly than DGI-I affected dentin. Reactivity of type VI collagen was significantly lower in normal teeth than in dentin from DGI-I affected patients (P<0.05). Expressions of dentin matrix protein (DMP)-1 and osteopontin (OPN) were observed in both normal dentin and dentin from DGI-I affected patients, without significant differences, being DMP1 generally more abundantly expressed. Immunolabeling for chondroitin sulfate (CS) and biglycan (BGN) was weaker in dentin from DGI-I-affected patients compared to normal dentin, this decrease being significant only for CS. This study shows ultrastructural alterations in dentin obtained from patients affected by DGI-I, supported by immunocytochemical assays of different collagenous and non-collagenous proteins.

Downloads

Download data is not yet available.
Published
2014-12-01
Section
Original Papers
Keywords:
Osteogenesis imperfecta, dentinogenesis imperfect, immune electron microscopy, collagen, non-collagenous proteins.
Statistics
Abstract views: 1163

PDF: 513
HTML: 525
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Orsini, G., Majorana, A., Mazzoni, A., Putignano, A., Falconi, M., Polimeni, A., & Breschi, L. (2014). Immunocytochemical detection of dentin matrix proteins in primary teeth from patients with dentinogenesis imperfecta associated with osteogenesis imperfecta. European Journal of Histochemistry, 58(4). https://doi.org/10.4081/ejh.2014.2405

Most read articles by the same author(s)