Internalized chitosan nanoparticles persist for long time in cultured cells

  • M. Malatesta | manuela.malatesta@univr.it University of Verona, Italy.
  • S. Grecchi University of Verona, Italy.
  • E. Chiesa University of Pavia, Italy.
  • B. Cisterna University of Verona, Italy.
  • M. Costanzo University of Verona, Italy.
  • C. Zancanaro University of Verona, Italy.

Abstract

Chitosan-based nanoparticles (chiNPs) are considered to be potentially good carriers for the sustained intracellular delivery of specific molecules. However, scarce attention has been paid to the long-lasting permanence of these NPs in the intracellular milieu, as well as to their intracellular fate (i.e., distribution, interaction with cell organelles, and degradation) in the long term. In the present study, the presence and subcellular location of FITC-labelled chiNPs were monitored in HeLa cells up to 14 days post-administration using multicolor-fluorescence confocal microscopy and diaminobenzidine photo-oxidation at transmission electron microscopy. The main result of the present study is the demonstration that internalized chiNPs persist inside the cell up to two weeks, occurring in both the cytoplasm and nucleus; accordingly, chiNPs are able to pass from mother to daughter cells through several mitotic cycles. The cells did not show increased mortality or structural damage up to 14 days after chiNP exposure.

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Author Biographies

M. Malatesta, University of Verona
Department of Neurological and Movement Sciences, Anatomy and Histology Section
S. Grecchi, University of Verona
Department of Neurological and Movement Sciences, Anatomy and Histology Section
E. Chiesa, University of Pavia
Department of Drugs Sciences
B. Cisterna, University of Verona
Department of Neurological and Movement Sciences, Anatomy and Histology Section
M. Costanzo, University of Verona
Department of Neurological and Movement Sciences, Anatomy and Histology Section
C. Zancanaro, University of Verona
Department of Neurological and Movement Sciences, Anatomy and Histology Section
Published
2015-02-18
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Section
Brief Reports
Keywords:
Nanoparticles, endosomal escape, cell nucleus, DAB photo-oxidation.
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How to Cite
Malatesta, M., Grecchi, S., Chiesa, E., Cisterna, B., Costanzo, M., & Zancanaro, C. (2015). Internalized chitosan nanoparticles persist for long time in cultured cells. European Journal of Histochemistry, 59(1). https://doi.org/10.4081/ejh.2015.2492