Cartilage canals in newborn dogs: histochemical and immunohistochemical findings

  • A. Di Giancamillo Department of Health, Animal Science and Food Safety, University of Milan, Italy.
  • M.E. Andreis Department of Health, Animal Science and Food Safety, University of Milan, Italy.
  • P. Taini Department of Health, Animal Science and Food Safety, University of Milan, Italy.
  • M.C. Veronesi Department of Veterinary Medicine, University of Milan, Italy.
  • M. Di Giancamillo Department of Veterinary Medicine, University of Milan, Italy.
  • S.C. Modina | silvia.modina@unimi.it Department of Health, Animal Science and Food Safety, University of Milan, Italy. http://orcid.org/0000-0002-2780-5971

Abstract

Cartilage canals (CCs) are microscopic structures involved in secondary ossification centers (SOCs) development. The features of CCs were investigated in the humeral and femoral proximal epiphyses of small-sized newborn dogs (from premature to 28 days after birth) with histochemical and immunohistochemical approaches. Masson’s Trichrome revealed a ring-shaped area around CCs, which changes in colour from green (immature collagen) to red (mature collagen) as ossification progresses; perichondrium staining always matched the ring colour. Safranin-O was always negative. Immunohistochemical analysis revealed immunopositivity for both collagen type I and V around the CCs; collagen type II was negative. CCs count showed a tendency to be higher in the humerus than in the femur. This work enlightened for the first time changes in composition of CCs surrounding matrix during SOCs development in dogs, paving the way to further investigations.

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Author Biographies

A. Di Giancamillo, Department of Health, Animal Science and Food Safety, University of Milan
Department of Health, Animal Science and Food Safety
M.E. Andreis, Department of Health, Animal Science and Food Safety, University of Milan
Department of Health, Animal Science and Food Safety
P. Taini, Department of Health, Animal Science and Food Safety, University of Milan
Department of Health, Animal Science and Food Safety
M.C. Veronesi, Department of Veterinary Medicine, University of Milan
Department of Veterinary Medicine
M. Di Giancamillo, Department of Veterinary Medicine, University of Milan
Department of Veterinary Medicine
S.C. Modina, Department of Health, Animal Science and Food Safety, University of Milan
Department of Health, Animal Science and Food Safety
Published
2016-09-15
Section
Original Papers
Keywords:
Cartilage canals, secondary ossification center, collagens, new born small sized dog.
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How to Cite
Di Giancamillo, A., Andreis, M., Taini, P., Veronesi, M., Di Giancamillo, M., & Modina, S. (2016). Cartilage canals in newborn dogs: histochemical and immunohistochemical findings. European Journal of Histochemistry, 60(3). https://doi.org/10.4081/ejh.2016.2701