3-hydroxi-anthranilic acid is early expressed in stroke

  • A. Mangas | amangas@gemacbio.com Gemacbio - Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT) - University of Salamanca, France.
  • J. Yajeya University of Salamanca, Department of Physiology, Spain.
  • N. González Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT), France.
  • I. Ruiz Gemacbio, France.
  • M. Geffard Gemacbio - Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT), France.
  • R. Coveñas University of Salamanca, Institute of Neurosciences of Castilla y León (INCYL), Laboratory of Neuroanatomy of the Peptidergic Systems, Spain.

Abstract

Using an immunohistochemical technique, we have studied the distribution of 3-OH-anthranilic acid (3-HAA) in the rat brain. Our study was carried out in control animals and in rats in which a stroke model (single transient middle cerebral artery occlusion) was performed. A monoclonal antibody directed against 3-HAA was also developed. 3-HAA was exclusively observed in the infarcted regions (ipsilateral striatum/cerebral cortex), 2, 5 and 21 days after the induction of stroke. In control rats and in the contralateral side of the stroke animals, no immunoreactivity for 3-HAA was visualized. Under pathological conditions (from early phases of stroke), we reported for the first time the presence of 3-HAA in the mammalian brain. By double immunohistochemistry, the coexistence of 3-HAA and GFAP was observed in astrocytes. The distribution of 3-HAA matched perfectly with the infarcted regions. Our findings suggest that, in stroke, 3-HAA could be involved in the tissue damage observed in the infarcted regions, since it is well known that 3-HAA exerts cytotoxic effects.

Downloads

Download data is not yet available.

Author Biographies

A. Mangas, Gemacbio - Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT) - University of Salamanca
Research Department Chief
Antibodies Department Manager
J. Yajeya, University of Salamanca, Department of Physiology

School of Medicine, Department of Physiology

Professor

N. González, Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT)

Research Dept

Technician

I. Ruiz, Gemacbio

Research Dept

Technician

 

M. Geffard, Gemacbio - Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT)

Chief Scientific Officer

Published
2016-12-02
Section
Original Papers
Keywords:
Astrocyte, ischemia, glia, immunohistochemistry, monoclonal antibody, neurotoxicity, 3-OH-anthranilic acid.
Statistics
Abstract views: 537

PDF: 171
HTML: 298
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Mangas, A., Yajeya, J., González, N., Ruiz, I., Geffard, M., & Coveñas, R. (2016). 3-hydroxi-anthranilic acid is early expressed in stroke. European Journal of Histochemistry, 60(4). https://doi.org/10.4081/ejh.2016.2709