Characterization of the role of RILP in cell migration

  • Azzurra Margiotta University of Salento, Department of Biological and Environmental Sciences and Technologies, Italy.
  • Cinzia Progida University of Oslo, Department of Biosciences, Centre for Immune Regulation, Norway.
  • Oddmund Bakke University of Oslo, Department of Biosciences, Centre for Immune Regulation, Norway.
  • Cecilia Bucci | cecilia.bucci@unisalento.it University of Salento, Department of Biological and Environmental Sciences and Technologies, Italy. http://orcid.org/0000-0002-6232-6183

Abstract

Rab-interacting lysosomal protein (RILP) is a regulator of late stages of endocytosis. Recent work proved that depletion of RILP promotes migration of breast cancer cells in wound healing assay, whereas its overexpression influences re-arrangements of actin cytoskeleton. Here, we further characterized the role of RILP in cell migration by analyzing several aspects of this process. We showed that RILP is fundamental also for migration of lung cancer cells regulating cell velocity. RILP silencing did not affect Golgi apparatus nor microtubules reorientation during migration. However, both RILP over-expression and expression of its mutated form, RILP-C33, impair cell adhesion and spreading. In conclusion, our results demonstrate that RILP has important regulatory roles in cell motility affecting migration velocity but also in cell adhesion and cell spreading.

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Author Biographies

Azzurra Margiotta, University of Salento, Department of Biological and Environmental Sciences and Technologies
Post-doc at at the Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy
Cinzia Progida, University of Oslo, Department of Biosciences, Centre for Immune Regulation
Senior Researcher at the University of Oslo, Oslo, Norway
Oddmund Bakke, University of Oslo, Department of Biosciences, Centre for Immune Regulation
Full professor at the University of Oslo, Oslo, Norway
Cecilia Bucci, University of Salento, Department of Biological and Environmental Sciences and Technologies
Full professor of Cell Biology at the University of Salento, Lecce, Italy
Published
2017-05-31
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Original Papers
Supporting Agencies
AIRC (Grant IG19068 to CB), Telethon Italy (Grant GGP16037 to CB), Norwegian Cancer Society (Grants 5760850 to CP and 4604944 to OB), Research Council of Norway (grants 239903 to CP, 230779 to OB, and Centre of Excellence funding project 179573)
Keywords:
RILP, cell migration, cell adhesion, microtubules, cell polarization.
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How to Cite
Margiotta, A., Progida, C., Bakke, O., & Bucci, C. (2017). Characterization of the role of RILP in cell migration. European Journal of Histochemistry, 61(2). https://doi.org/10.4081/ejh.2017.2783