Characterization of the role of RILP in cell migration

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Azzurra Margiotta
Cinzia Progida
Oddmund Bakke
Cecilia Bucci *
(*) Corresponding Author:
Cecilia Bucci | cecilia.bucci@unisalento.it

Abstract

Rab-interacting lysosomal protein (RILP) is a regulator of late stages of endocytosis. Recent work proved that depletion of RILP promotes migration of breast cancer cells in wound healing assay, whereas its overexpression influences re-arrangements of actin cytoskeleton. Here, we further characterized the role of RILP in cell migration by analyzing several aspects of this process. We showed that RILP is fundamental also for migration of lung cancer cells regulating cell velocity. RILP silencing did not affect Golgi apparatus nor microtubules reorientation during migration. However, both RILP over-expression and expression of its mutated form, RILP-C33, impair cell adhesion and spreading. In conclusion, our results demonstrate that RILP has important regulatory roles in cell motility affecting migration velocity but also in cell adhesion and cell spreading.


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Author Biographies

Azzurra Margiotta, University of Salento, Department of Biological and Environmental Sciences and Technologies

Post-doc at at the Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy

Cinzia Progida, University of Oslo, Department of Biosciences, Centre for Immune Regulation

Senior Researcher at the University of Oslo, Oslo, Norway

Oddmund Bakke, University of Oslo, Department of Biosciences, Centre for Immune Regulation

Full professor at the University of Oslo, Oslo, Norway

Cecilia Bucci, University of Salento, Department of Biological and Environmental Sciences and Technologies

Full professor of Cell Biology at the University of Salento, Lecce, Italy