Analysis of mineral apposition rates during alveolar bone regeneration over three weeks following transfer of BMP-2/7 gene via in vivo electroporation

  • Mariko Kawai | kawai-m@cc.osaka-dent.ac.jp Osaka Dental University, Department of Pharmacology, Japan.
  • Yohei Kataoka Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Department of Oral Morphology, Japan.
  • Junya Sonobe Department of Oral and Maxillofacial Surgery, Kyoto University, Japan.
  • Hiromitsu Yamamoto Department of Oral and Maxillofacial Surgery, Kyoto University, Japan.
  • Hiroki Maruyama Niigata University Graduate School of Medicine and Dental Sciences, Department of Clinical Nephroscience, Japan.
  • Toshio Yamamoto Department of Oral Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.
  • Kazuhisa Bessho Department of Oral and Maxillofacial Surgery, Kyoto University, Japan.
  • Kiyoshi Ohura Department of Pharmacology, Osaka Dental University, Japan.

Abstract

Alveolar bone is not spontaneously regenerated following trauma or periodontitis. We previously proposed an animal model for new alveolar bone regeneration therapy based on the non-viral BMP-2/7 gene expression vector and in vivo electroporation, which induced the formation of new alveolar bone over the course of a week. Here, we analysed alveolar bone during a period of three weeks following gene transfer to periodontal tissue. Non-viral plasmid vector pCAGGS-BMP-2/7 or pCAGGS control was injected into palatal periodontal tissue of the first molar of the rat maxilla and immediately electroporated with 32 pulses of 50 V for 50 msec. Over the following three weeks, rats were double bone-stained by calcein and tetracycline every three days and mineral apposition rates (MAR) were measured. Double bone-staining revealed that MAR of alveolar bone was as similar level three days before BMP-2/7 gene transfer as three days after gene transfer. However, from 3 to 6 days, 6 to 9 days, 9 to 12 days, 12 to 15 days, 15 to 18 days, and 18 to 20 days after, MARs were significantly higher than prior to gene transfer. Our proposed gene therapy for alveolar bone regeneration combining non-viral BMP-2/7 gene expression vector and in vivo electroporation could increase alveolar bone regeneration potential in the targeted area for up to three weeks.

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Published
2018-08-09
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Original Papers
Keywords:
BMP, gene transfer, in vivo electroporation, alveolar bone, histomorphometry
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How to Cite
Kawai, M., Kataoka, Y., Sonobe, J., Yamamoto, H., Maruyama, H., Yamamoto, T., Bessho, K., & Ohura, K. (2018). Analysis of mineral apposition rates during alveolar bone regeneration over three weeks following transfer of BMP-2/7 gene via in vivo electroporation. European Journal of Histochemistry, 62(3). https://doi.org/10.4081/ejh.2018.2947