Co-transplantation of bone marrow mesenchymal stem cells and monocytes in the brain stem to repair the facial nerve axotomy

  • Li Wu Department of Medical Imaging, First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Dan Han Department of Medical Imaging, First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Jie Jiang Department of Medical Imaging, First Affiliated Hospital of Kunming Medical University, Kunming, China. https://orcid.org/0000-0002-1658-4544
  • Xiaojie Xie Department of Medical Imaging, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China, China.
  • Xunran Zhao Department of Medical Imaging, First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Tengfei Ke Department of Medical Imaging, Third Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Wen Zhao Department of Medical Imaging, First Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Liu Liu | liuliuwuli@126.com Department of Plastic Surgery, First Affiliated Hospital of Kunming Medical University, Kunming, China. https://orcid.org/0000-0001-9724-4295
  • Wei Zhao Department of Medical Imaging, First Affiliated Hospital of Kunming Medical University, Kunming, China.

Abstract

After the facial nerve axotomy (FNA), the distal end of the axon would gradually decay and disappear. Accumulated evidence shows that transplantation of bone marrow mesenchymal stem cells (BMSCs) reveals potential in the treatment of nervous system diseases or injuries. This study is aimed at investigating the therapeutic effects of co-transplantation of BMSCs and monocytes in FNA. We found that co-culture significantly elevated the CD4+/CD8+ ratio and CD4+ CD25+ T cell proportion compared with monocytes transplantation, and enhanced the differentiation of BMSCs into neurons. After the cell transplantation, the lowest apoptosis in the facial nerve nucleus was found in the co-transplantation group 2 (BMSCs:monocytes= 1:30). Moreover, the lowest expression levels of pro-inflammatory cytokines and the highest expression levels of anti-inflammatory cytokines were observed in the co-transplantation group 2 (BMSCs: monocytes= 1:30). The highest expression levels of protein in the JAK/STAT6 pathway and the SDF-1/CXCR4 axis were found in the co-transplantation group 2. BMSC/monocyte co-transplantation significantly improves the microenvironment in the facial nerve nucleus in FNA rats; therefore these findings suggest that it could promote the anti-/pro-inflammatory balance shift towards the anti-inflammatory microenvironment, alleviating survival conditions for BMSCs, regulating BMSC the chemotaxis homing, differentiation, and the section of BMSCs, and finally reducing the neuronal apoptosis. These findings might provide essential evidence for the in-hospital treatment of FNA with co-transplantation of BMSCs and monocytes.

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Published
2020-06-19
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Issue
Section
Special Issue - Stem cells and regenerative medicine
Supporting Agencies
National Natural Science Foundation of China Grant (No. 81460298), Yunnan Provincial Science and Technology Department-Kunming Medical University Applied Basic Research Joint Special Fund Grant (No. 2015FB038)
Keywords:
Facial nerve axotomy (FNA), bone marrow mesenchymal stem cells (BMSCs), monocytes, co-transplantation, microenvironment
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How to Cite
Wu, L., Han, D., Jiang, J., Xie, X., Zhao, X., Ke, T., Zhao, W., Liu, L., & Zhao, W. (2020). Co-transplantation of bone marrow mesenchymal stem cells and monocytes in the brain stem to repair the facial nerve axotomy. European Journal of Histochemistry, 64(s2). https://doi.org/10.4081/ejh.2020.3136