Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii)

Histological structure of Rana dybowskii oviducts in the breeding period
Submitted: 11 June 2019
Accepted: 4 September 2019
Published: 25 September 2019
Abstract Views: 586
PDF: 332
HTML: 12
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

One special physiological phenomenon of the Chinese brown frog (Rana dybowskii) is that its oviduct goes through expansion prior to pre-hibernation instead of expanding during the breeding period. Our previous study discovered that some cytokines such as interleukin-1 beta (IL-1β) and its receptor type I (IL1R1) proteins were expressed in the oviduct of Rana dybowskii. In this study, we continued to investigate the localizations and expression levels of cytokines including toll-like receptor 4 (TLR4) and its related factors, myeloid differentiation factor 88 (MyD88), interleukin-1 receptor-associated kinase (IRAK) and nuclear factor kappa B (NF-κΒ) in the oviduct of Rana dybowskii during the breeding period and pre-hibernation. Morphological results showed that there were significant differences in oviductal weight and pipe diameter with significantly higher values in pre-hibernation than those of the breeding period. Strong immunostaining of TLR4, MyD88, IRAK4 and NF-κΒ were observed in the pre-hibernation. These proteins levels had no significant difference between these two periods except MyD88 which was significantly higher in the pre-hibernation. The mRNA expression levels of MyD88 and NF-κΒ were obviously higher in pre-hibernation than those of the breeding period. These findings suggested that TLR4/MyD88 signal pathway might participate in the oviductal functions of Rana dybowskii during the breeding period and pre-hibernation.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Supporting Agencies

Beijing Natural Science Foundation (8182039), National Natural Science Foundation of China (31872320, 21806010), Young Scientist Start-up funding of Beijing Forestry University (BLX201714)

How to Cite

Zhang, Y., Zhang, A., Zhao, Y., Feng, X., Sheng, Y., Zhang, H., Weng, Q., & Xu, M. (2019). Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii). European Journal of Histochemistry, 63(3). https://doi.org/10.4081/ejh.2019.3050