https://doi.org/10.4081/ejh.2021.3247

MicroRNA-29b-3p promotes 5-fluorouracil resistance via suppressing TRAF5-mediated necroptosis in human colorectal cancer

Vol. 65 No. 2 (2021)
Submitted: 16 March 2021
Accepted: 21 May 2021
Published: 22 June 2021
colorectal cancer, miR-29-3p, TRAF5, necroptosis, 5-fluorouracil resistance
Abstract Views: 1113
PDF: 765
Supplementary: 138
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Authors

Shuimei Wu
Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.
Yun Zhou
Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.
Ping Liu
Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.
Hui Zhang
Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.
Wanliang Wang
Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.
Yuan Fang
Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.
Xiang Shen
hao350318178@outlook.com
https://orcid.org/0000-0003-0702-6847
Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.

Drug resistance in colorectal cancer is a great challenge in clinic. Elucidating the deep mechanism underlying drug resistance will bring much benefit to diagnosis, therapy and prognosis in patients with colorectal cancer. In this study, miR-29b-3p was shown to be involved in resistance to 5-fluorouracil (5-FU)-induced necroptosis of colorectal cancer. Further, miR-29b-3p was shown to target a regulatory subunit of necroptosis TRAF5. Rescue of TRAF5 could reverse the effect of miR-29b-3p on 5-FU-induced necroptosis, which was consistent with the role ofnecrostatin-1 (a specific necroptosis inhibitor). Then it was demonstrated that miR-29b-3p was positively correlated with chemo-resistance in colorectal cancer while TRAF5 negatively. In conclusion, it is deduced that miR-29b-3p/TRAF5 signaling axis plays critical role in drug resistance in chemotherapy for colorectal cancer patients by regulating necroptosis. The findings in this study provide us a new target for interfere therapy in colorectal cancer.

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How to Cite

Wu, S., Zhou, Y., Liu, P., Zhang, H., Wang, W., Fang, Y., & Shen, X. (2021). MicroRNA-29b-3p promotes 5-fluorouracil resistance <em>via</em> suppressing TRAF5-mediated necroptosis in human colorectal cancer . European Journal of Histochemistry, 65(2). https://doi.org/10.4081/ejh.2021.3247

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