P35 | EARLY LIFE STRESS CAN CAUSE CHANGES IN THE HIPPO SIGNALING PATHWAY IN RAT OVARIES

İ. İnanç¹, E. Baysal¹, O. Bender², A. Atalay², E. Erdemli¹ | ¹Ankara University, Faculty of Medicine, Department of Histology and Embryology, Ankara, Türkiye; ²Ankara University, Biotechnology Institute, Ankara, Türkiye

Hippo signaling pathway has been reported that it participates in many events such as cell division, proliferation, differentiation, and programmed cell death. YAP (Yes-Associated Protein) is one of the key components of this pathway¹. It has been reported that early life stress causes oxidative stress in the mouse ovary which can affect follicle development². However, the molecular mechanisms underlying these problems caused by early life stress have not yet been elucidated. 180-min maternal deprivation and isolation model was applied to the experimental group every day between the 1st and 14th postnatal day³. In total of 6 newborn Wistar albino female rats were used as control (n=3) and experimental (n=3) groups. Tail blood was collected to determine the serum corticosterone level before and after stress conditions on 14th postnatal day whether early life stress had developed. The groups were sacrificed on the 55th postnatal day. The YAP expression level was evaluated for immunohistochemical analyses. When compared to the control group, the serum corticosterone levels were high (p<0.05) and YAP expressions increased in granulosa cells and corpus luteum of the experimental group (p<0.05). This study suggests that stress created in the early period may cause various errors as the ovaries enter the adulthood period. It is also thought that the Hippo pathway plays an active role in this process.