Articles
Vol. 70 No. 3 (2026)
https://doi.org/10.4081/ejh.2026.4606

SPRR2B knockdown inhibits the proliferation and inflammatory response in M5-treated human HaCaT keratinocytes

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Published: 6 August 2026
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psoriasis, SPRR2B, HaCaT, JAK1, MDM2, p53

Authors

Zhenzhen Ding
Department of Dermatology, The Affiliated Yangming Hospital of Ningbo University (Yuyao People's Hospital), Yuyao, China.
Chengchi Wang
Department of Dermatology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Mingfeng Lv
lvmingfen1106@163.com
Department of Dermatology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Psoriasis is a chronic inflammatory skin disease characterized by keratinocyte hyperproliferation and excessive immune activation, for which curative therapies remain limited. This study aimed to investigate the role of small proline-rich protein 2B (SPRR2B) in psoriasis pathogenesis. In this study, gene Expression Omnibus (GEO) dataset GSE13355 was analyzed using R Language. Cell proliferation, gene and protein expression were measured with CCK8, RT-qPCR and immunofluorescence staining, respectively. Bioinformatics analysis of the GSE13355 dataset revealed that SPRR2B was significantly overexpressed in psoriatic lesions and was positively correlated with immune cell infiltration and multiple inflammatory pathways. In an M5-induced HaCaT keratinocyte psoriatic model, SPRR2B knockdown markedly suppressed cell proliferation and promoted apoptosis via regulation of the MDM2/p53/CDKN1A axis. Furthermore, SPRR2B knockdown reduced the secretion of IL-6, IFN-γ, TNF-α, and IL-1β, and inhibited JAK1 phosphorylation. These findings demonstrated that SPRR2B promotes keratinocyte proliferation and inflammatory response in psoriasis, suggesting it may serve as a promising diagnostic biomarker and therapeutic target for the disease.

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CRediT authorship contribution

Zhenzhen Ding, conceptualization, methodology, investigation, supervision, writing – original draft. Mingfen Lv, conceptualization, methodology, supervision, validation, data curation, writing – original draft. Chengchi Wang, investigation, data curation, formal analysis. All authors have read and approved the final version of the manuscript.

Supporting Agencies

The First Affiliated Hospital of Wenzhou Medical University

Data Availability Statement

The data used to support the findings of this study are available from the corresponding author on reasonable request.

How to Cite



1.
Ding Z, Wang C, Lv M. SPRR2B knockdown inhibits the proliferation and inflammatory response in M5-treated human HaCaT keratinocytes. Eur J Histochem [Internet]. 2026 Aug. 6 [cited 2026 Sep. 4];70(3). Available from: https://www.ejh.it/ejh/article/view/4606
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