https://doi.org/10.4081/ejh.2023.3746

Substance P is overexpressed in cervical squamous cell carcinoma and promoted proliferation and invasion of cervical cancer cells in vitro

Vol. 67 No. 3 (2023)
Submitted: 6 April 2023
Accepted: 20 July 2023
Published: 31 July 2023
Substance P, cervical cancer, proliferation, invasion, ERK, MMP9
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Authors

Ying Wang
Department of Gynecology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
Shifa Yuan
Department of General Surgery, Hospital of Hebei Province Crop of Chinese Armed Police Force, Shijiazhuang, China.
Jing Ma
shj911la@gmail.com
https://orcid.org/0000-0003-4610-350X
Department of Gynecology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
Hong Liu
Department of Gynecology Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
Lizhen Huang
Department of Gynecology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
Fengzhen Zhang
Department of Gynecology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.

This study aimed to investigate the expression and function of substance P in cervical squamous cell carcinoma. Cancer tissues and adjacent tissues of 20 patients with cervical squamous cell carcinoma in our hospital were collected. The expression of substance P was detected by immunohistochemistry and Western blot analysis. Cervical squamous cell carcinoma line SiHa was treated with different concentrations of substance P. The proliferation of SiHa cells was detected by EdU assay, and the invasion ability of SiHa cells was detected by transwell assay. The phosphorylation of ERK1/2 and the expression of MMP9 were detected by Western blot analysis. The results showed that substance P was expressed in the cytoplasm and some cell membranes of cervical squamous cell carcinoma cells. The expression of substance P in cervical cancer tissues was significantly higher than that in the adjacent tissues. Compared with the control group, substance P significantly promoted the proliferation and invasion of SiHa cells in a concentration dependent manner and activated the phosphorylation of ERK1/2 and upregulated the expression of MMP9 in SiHa cells. In conclusion, substance P is highly expressed in cervical squamous cell carcinoma and can promote cervical cancer cell proliferation and invasion. The mechanism is related to the activation of ERK1/2 pathway to upregulate MMP9.

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Ethics Approval

This study was approved by the Ethics Committee of the Fourth Hospital of Hebei Medical University (approval no. 20190312).

Supporting Agencies

Hebei Province Medical Science Research Project Program

How to Cite

Wang, Y., Yuan, S., Ma, J., Liu, H., Huang, L., & Zhang, F. (2023). Substance P is overexpressed in cervical squamous cell carcinoma and promoted proliferation and invasion of cervical cancer cells <em>in vitro</em>. European Journal of Histochemistry, 67(3). https://doi.org/10.4081/ejh.2023.3746
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