Brief Reports
Vol. 69 No. 4 (2025)
https://doi.org/10.4081/ejh.2025.4244

Immunohistochemical analysis of YB-1 expression in the developing mouse eye

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Published: 3 November 2025
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eye, development, immunohistochemistry, cornea, retina, YB-1

Authors

Alexander Forian Nass
Brandenburg Medical School Theodor Fontane (MHB), University Hospital Brandenburg/Havel, Institute of Pathology, Brandenburg/Havel, Germany.
Hella Wolf
Institute of Pathology, Otto von Guericke University, Magdeburg, Germany.
Saadettin Sel
Department of Ophthalmology, University of Heidelberg, Germany.
Thomas Kalinski
Brandenburg Medical School Theodor Fontane (MHB), University Hospital Brandenburg/Havel, Institute of Pathology, Brandenburg/Havel, Germany.
Norbert Nass
norbert.nass@mhb-fontane.de
Brandenburg Medical School Theodor Fontane (MHB), University Hospital Brandenburg/Havel, Institute of Pathology, Brandenburg/Havel; Institute of Pathology, Otto von Guericke University, Magdeburg, Germany.

Cold shock domain (CSD) proteins, such as YB-1, play a crucial role in the regulation of transcription, mRNA stability, and translation. Consequently, YB-1 is implicated in processes such as cell differentiation, oncogenesis and oxidative stress response. The development of the eye is a complex process that involves the differentiation of numerous highly specialized cell types. We hypothesized that YB-1 is involved in both eye development and stress defense mechanisms. As an initial step, we investigated the expression of YB-1 during the embryology of the mouse eye. YB-1 mRNA could be detected by RT-PCR and sequencing the PCR product in retinal tissue of adult mice. To elucidate the expression pattern of YB-1 protein during mouse eye development, we analyzed its expression in the developing mouse eye at embryonic day 13 (E13), E15, E18 and postnatal day 14 (P14) using immunohistochemistry. Expression of the YB-1 protein was detected in all retinal cells, as well as in the corneal and lens epithelial cells, throughout all stages of eye development examined. These findings suggest that YB-1 could have a significant role in the eye, potentially related to development and differentiation.

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Supporting Agencies

Ministry of Science, Research and Cultural Affairs of the State of Brandenburg

How to Cite



1.
Nass AF, Wolf H, Sel S, Kalinski T, Nass N. Immunohistochemical analysis of YB-1 expression in the developing mouse eye. Eur J Histochem [Internet]. 2025 Nov. 3 [cited 2025 Dec. 26];69(4). Available from: https://www.ejh.it/ejh/article/view/4244
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