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Walking a thin line between fixation and epitope binding – characterization of antigen retrieval methods suitable for eosinophil and HSV-2 staining in formalin-fixed female reproductive tissue

Lisa Marie Wadephul, Kathrin Arndts, Gnatoulma Katawa, Eva Dietlmeier, William Horsnell, Achim Hoerauf, Manuel Ritter

16-04-2024

https://doi.org/10.4081/ejh.2024.3929

1595

PDF: 538

HTML: 17
Three-dimensional scaffold-free fusion culture: the way to enhance chondrogenesis of in vitro propagated human articular chondrocytes

M. Lehmann, F. Martin, K. Mannigel, K. Kaltschmidt, U. Sack, U. Anderer

e31

05-11-2013

https://doi.org/10.4081/ejh.2013.e31

4832

PDF: 1061

HTML: 965
Leptin affects filopodia and cofilin in NK-92 cells in a dose- and time-dependent manner

Jana Oswald, Maximilian Büttner, Simon Jasinski-Bergner, Roland Jacobs, Philip Rosenstock, Heike Kielstein

26-01-2018

https://doi.org/10.4081/ejh.2018.2848

3312

PDF: 1198

HTML: 114
Hsp10, Hsp70, and Hsp90 immunohistochemical levels change in ulcerative colitis after therapy

G. Tomasello, C. SciumÃ©, F. Rappa, V. Rodolico, M. Zerilli, A. Martorana, G. Cicero, R. De Luca, P. Damiani, F.M. Accardo, M. Romeo, F. Farina, G. Bonaventura, G. Modica, G. Zummo, E. Conway de Macario, A.J.L. Macario, F. Cappello

e38

24-10-2011

https://doi.org/10.4081/ejh.2011.e38

2041

PDF: 834
Low ozone concentrations stimulate cytoskeletal organization, mitochondrial activity and nuclear transcription

M. Costanzo, B. Cisterna, A. Vella, T. Cestari, V. Covi, G. Tabaracci, M. Malatesta

21-04-2015

https://doi.org/10.4081/ejh.2015.2515

3059

PDF: 1000

HTML: 410
Secretion of immunomodulating neuropeptides (VIP, SP) and nitric oxide synthase in porcine small intestine during postnatal development

A. Kovsca Janjatovic, H. Valpotic, D. Kezic, G. LackoviÄ‡, G. Gregorovic, S. Sladoljev, G. MrÅ¡iÄ‡, M. Popovic, I. Valpotic

e30

13-09-2012

https://doi.org/10.4081/ejh.2012.e30

1346

PDF: 780
dCTP pyrophosphohydrase exhibits nucleic accumulation in multiple carcinomas

Y. Zhang, W.Y. Ye, J.Q. Wang, S.J. Wang, P. Ji, G.Y. Zhou, G.P. Zhao, H.L. Ge, Y. Wang

e29

25-09-2013

https://doi.org/10.4081/ejh.2013.e29

2993

PDF: 810

HTML: 419
Porphyromonas gingivalis lipopolysaccharide stimulation in human periodontal ligament stem cells: role of epigenetic modifications to the inflammation

Francesca Diomede, Soundara Rajan Thangavelu, Ilaria Merciaro, Monica D'Orazio, Placido Bramanti, Emanuela Mazzon, Oriana Trubiani

25-08-2017

https://doi.org/10.4081/ejh.2017.2826

3069

PDF: 1291

HTML: 140
Effects of hypophyseal or thymic allograft on thymus development in partially decerebrate chicken embryos: expression of PCNA and CD3 markers

M. Aita, F. Benedetti, E. Carafelli, E. Caccia, N. Romano

e37

30-08-2010

https://doi.org/10.4081/ejh.2010.e37

1205

PDF: 750

HTML: 3927
Light microscopy - Methods and protocols

CarloAlberto Redi

br15

24-11-2011

https://doi.org/10.4081/ejh.2011.br15

990

PDF: 475
Expressions of IL-6, TNF-α and NF-κB in the skin of Chinese brown frog (Rana dybowskii)

Liqin Xi, Chen Wang, Pengyu Chen, Qi Yang, Ruiqi Hu, Haolin Zhang, Qiang Weng, Meiyu Xu

19-10-2017

https://doi.org/10.4081/ejh.2017.2834

2415

PDF: 873

HTML: 64
Colorimetric and fluorescent TRAP assays for visualising and quantifying fish osteoclast activity

Lalith Prabha Ethiraj, En Lei Samuel Fong, Ranran Liu, Madelynn Chan, Christoph Winkler, Tom James Carney

24-03-2022

https://doi.org/10.4081/ejh.2022.3369

2560

PDF: 1605

HTML: 94
A semiquantitative scoring tool to evaluate eccentric exercise-induced muscle damage in trained rats

D. Rizo-Roca, J.G. RÃos-KristjÃ¡nsson, C. NÃºÃ±ez-Espinosa, A. AscensÃ£o, J. MagalhÃ£es, J.R. Torrella, T. PagÃ¨s, G. Viscor

02-11-2015

https://doi.org/10.4081/ejh.2015.2544

2246

PDF: 859

HTML: 462
Influence of surgical and chemical orchidectomy on weight and distribution of AChE-nerve fibres in thymuses of adult rats

F. Dorko, D. KluchovÃ¡, A. BolekovÃ¡, K. LovasovÃ¡, T. Å pakovskÃ¡, T. BoroÅ¡ovÃ¡

e22

12-07-2011

https://doi.org/10.4081/ejh.2011.e22

1814

PDF: 533

HTML: 2515
RNA therapeutics - Function, design and delivery

Carlo Alberto Redi

br7

17-06-2011

https://doi.org/10.4081/ejh.2011.br7

921

PDF: 437

HTML: 172
Efficacy of immunohistochemical staining in detecting Helicobacter pylori in Saudi patients with minimal and atypical infection

Mohammed Akeel, Ahmed Elhafey , Atef Shehata , Erwa Elmakki , Thanaa Aboshouk, Hussein Ageely , Mohammed Salih Mahfouz

20-07-2021

https://doi.org/10.4081/ejh.2021.3222

3008

PDF: 988

HTML: 305
Spatio-temporal course of macrophage-like cell accumulation after experimental embolic stroke depending on treatment with tissue plasminogen activator and its combination with hyperbaric oxygenation

D. Michalski, M. Heindl, J. Kacza, F. Laignel, L. KÃ¼ppers-Tiedt, D. Schneider, J. Grosche, J. Boltze, M. LÃ¶hr, C. Hobohm, W. HÃ¤rtig

e14

09-05-2012

https://doi.org/10.4081/ejh.2012.14

6677

PDF: 784

HTML: 10450
Endothelial barrier antigen-immunoreactivity is conversely associated with blood-brain barrier dysfunction after embolic stroke in rats

J. Pelz, W. HÃ¤rtig, C. Weise, C. Hobohm, D. Schneider, M. Krueger, J. Kacza, D. Michalski

e38

18-12-2013

https://doi.org/10.4081/ejh.2013.e38

3423

PDF: 834

HTML: 232
Non-invasive imaging demonstrates clinical features of ankylosing spondylitis in a rat adjuvant model: a case study

N. Accart, J. Dawson, F. Kolbinger, I. Kramer, N. Beckmann

02-11-2016

https://doi.org/10.4081/ejh.2016.2667

2326

PDF: 886

HTML: 376
LOCUS COERULEUS STRUCTURAL INTEGRITY IS ASSOCIATED WITH CIRCULATING SOLUBLE AXL IN ALZHEIMER’S DISEASE Galgani A¹, Scotto M², Mary A³, Lombardo F⁴, Martini N⁴, Siciliano G⁵, Ceravolo R⁵, Heneka MT^3,6 and Giorgi FS^1,7 | ¹Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, Pisa, Italy; ²Laboratory of Medical Science, Institute of Life Sciences, Scuola Superiore Sant’Anna, Pisa, Italy; ³Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux, Luxembourg; ⁴Department of Radiology, Fondazione Toscana “G. Monasterio”, Pisa, Italy; ⁵Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy; ⁶Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts, USA; ⁷I.R.C.C.S. Stella Maris, Calambrone, Pisa, Italy

12-12-2025

https://doi.org/10.4081/ejh.2025.4467

289
Peribiliary gland damage due to liver transplantation involves peribiliary vascular plexus and vascular endothelial growth factor

Antonio Franchitto, Diletta Overi, Romina Mancinelli, Anna Paola Mitterhofer, Paolo Muiesan, Francesca Tinti, Ilaria Umbro, Stefan G. Hubscher, Paolo Onori, Eugenio Gaudio, Guido Carpino

10-05-2019

https://doi.org/10.4081/ejh.2019.3022

2445

PDF: 780

Supplementary: 279

HTML: 15
SerpinB3 administration protects liver against ischemia-reperfusion injury

Cristian Turato, Mariapia Vairetti, Marta Cagna, Alessandra Biasiolo, Andrea Ferrigno, Santina Quarta, Mariagrazia Ruvoletto, Silvia De Siervi, Patrizia Pontisso, Laura Giuseppina Di Pasqua

28-10-2022

https://doi.org/10.4081/ejh.2022.3561

1300

PDF: 750

HTML: 34
RXRα is upregulated in first trimester endometrial glands of spontaneous abortions unlike LXR and PPARγ

J. Knabl, A. Vattai, R. Hüttenbrenner, S. Hutter, M. Karsten, U. Jeschke

02-11-2016

https://doi.org/10.4081/ejh.2016.2665

2133

PDF: 1074

HTML: 319
A PILOT STUDY TO INVESTIGATE THE ANTIAGING EFFECTS OF THE OLIVE POMACE BY-PRODUCT ON THE BRAIN OF DROSOPHILA MELANOGASTER Makladissi S^1†, Tomassoni D^2†*, Nardin T³, Bellitto V², Cappelli A², Di Cara F^1‡ and Beghelli D^2‡ | ¹Department of Microbiology and Immunology, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada; ²School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, MC, Italy ; ³Chemical Unit Oenological Vines Agri-food - Technology Transfer Centre Foundation Edmund Mach, TN, Italy ^†These authors equally contributed. ^‡These authors share the authorship

12-12-2025

https://doi.org/10.4081/ejh.2025.4513

209
Porcine intestinal mast cells. Evaluation of different fixatives for histochemical staining techniques considering tissue shrinkage

J. Rieger, S. Twardziok, H. Huenigen, R. M. Hirschberg, J. Plendl

e21

30-07-2013

https://doi.org/10.4081/ejh.2013.e21

4236

PDF: 1140

HTML: 1300
Subcellular distribution and expression of cofilin and ezrin in human colon adenocarcinoma cell lines with different metastatic potential

D. Nowak, A. J. Mazur, A. Popow-WoÅºniak, A. RadwaÅ„ska, H. G. Mannherz, M. Malicka-BÅ‚aszkiewicz

e14

13-04-2010

https://doi.org/10.4081/ejh.2010.e14

1731

PDF: 636

HTML: 2677
PATHOMICS – A NOVEL OMICS APPROACH FOR HISTOPATHOLOGY P. Boor^1,2 | ¹Institute of Pathology, RWTH Aachen University Hospital, Aachen, Germany; Electron Microscopy Facility, RWTH Aachen University Hospital, Aachen, Germany; ²Division of Nephrology and Immunology, RWTH Aachen University Hospital, Aachen, Germany

21-08-2025

https://doi.org/10.4081/ejh.2025.4284

188
High expression of both desmoplastic stroma and epithelial to mesenchymal transition markers associate with shorter survival in pancreatic ductal adenocarcinoma

Damián Sánchez-Ramírez, Rafael Medrano-Guzmán, Fernando Candanedo-González, Jazmín De Anda-González, Luis Enrique García-Rios, Vadim Pérez-Koldenkova, Marcos Gutiérrez-de la Barrera, Sara Rodríguez-Enríquez, Marco Velasco-Velázquez, Silvia Cecilia Pacheco-Velázquez, Patricia Piña-Sánchez, Héctor Mayani, Alejandro Gómez-Delgado, Alberto Monroy-García, Ana Karen Martínez-Lara, Juan José Montesinos

17-02-2022

https://doi.org/10.4081/ejh.2022.3360

2515

PDF: 979

HTML: 40
Histomorphometric evaluation of intestinal cellular immune responses in pigs immunized with live oral F4ac+ non-enterotoxigenic E. coli vaccine against postweaning colibacillosis

A. KovÅ¡ca Janjatovic, G. LackoviÄ‡, F. Bozic, D. Kezic, M. Popovic, H. ValpotiÄ‡, I. Harapin, Z. Pavicic, B. Njari, I. ValpotiÄ‡

e4

11-02-2010

https://doi.org/10.4081/ejh.2010.e4

1694

PDF: 909

HTML: 1678
A single simple procedure for dewaxing, hydration and heat-induced epitope retrieval (HIER) for immunohistochemistry in formalin fixed paraffin-embedded tissue

I.M.S. Paulsen, H. Dimke, S. Frische

03-11-2015

https://doi.org/10.4081/ejh.2015.2532

3448

PDF: 1737

HTML: 804
HOST/PATHOGEN INTERACTIONS AND THE ROLE OF THE BARRIERS: ON THE EDGE BETWEEN HEALTH AND MULTIFACTORIAL/COMPLEX DISEASES G. Di Sante¹, A. M. Stabile¹, A. Pistilli¹, M. Ruggirello^1,2, F. Michetti^3,4, F. Ria², M. Rende¹ | ¹ Department of Surgery and Medicine, Sect. of Human, Clinical and Forensic Anatomy, University of Perugia, Perugia, Italy; ²Department of Translational Medicine and Surgery, Section of General Pathology, University “Cattolica del Sacro Cuore” of Rome, Roma, Italy; ³Department of Medicine, LUM University, Casamassima, Bari, Italy; ⁴Italy Genes, Rome, Italy

21-08-2025

https://doi.org/10.4081/ejh.2025.4314

264
Overview of the optical properties of fluorescent nanoparticles for optical imaging

Federico Boschi, Francesco De Sanctis

29-08-2017

https://doi.org/10.4081/ejh.2017.2830

1769

PDF: 804

HTML: 73
Restorative effects of camellia oil on the skin-barrier function in a model of DNCB-induced atopic dermatitis

Shicheng Jiao, Lijun Deng, Mu Niu, Jie Yang

21-01-2025

https://doi.org/10.4081/ejh.2025.4147

4190

PDF: 817

HTML: 52
Establishment and biological characteristics of a Jingning chicken embryonic fibroblast bank

C. Bai, D. Wang, C. Li, D. Jin, C. Li, W. Guan, Y. Ma

e4

29-03-2011

https://doi.org/10.4081/ejh.2011.e4

1862

PDF: 722

HTML: 6755
miR-26b regulates cell proliferation and apoptosis of CD117+CD44+ ovarian cancer stem cells by targeting PTEN

Zubiao Gao, Xiaofeng Ye, Anne Bordeaux, Stanka Hettich, Siyao Lin, Fang Han, Yan Jia

04-02-2021

https://doi.org/10.4081/ejh.2021.3186

2261

PDF: 723

HTML: 17
A simplified immunohistochemical classification of skeletal muscle fibres in mouse

M. Kammoun, I. Cassar-Malek, B. Meunier, B. Picard

24-06-2014

https://doi.org/10.4081/ejh.2014.2254

4733

PDF: 1709

HTML: 979
Co-overexpression of bcl-2 and c-myc in uterine cervix carcinomas and premalignant lesions

Z. Protrka, S. Arsenijevic, A. Dimitrijevic, S. Mitrovic, V. Stankovic, M. Milosavljevic, T. Kastratovic, J. Djuric

e8

16-03-2011

https://doi.org/10.4081/ejh.2011.e8

1103

PDF: 636

HTML: 3056
Expression of the HPV18/E6 oncoprotein induces DNA damage

Elva I. Cortés Gutiérrez, Catalina García-Vielma, Adriana Aguilar-Lemarroy, Veronica Vallejo-Ruíz, Patricia Piña-Sánchez, Pablo Zapata-Benavides, Jaime Gosalvez

15-06-2017

https://doi.org/10.4081/ejh.2017.2773

1908

PDF: 773

HTML: 211
NADPH-d activity in rat thymus after the application of retinoid acid

F. Dorko, T. Å pakovskÃ¡, K. LovasovÃ¡, P. PatleviÄ, D. KluchovÃ¡

e7

08-02-2012

https://doi.org/10.4081/ejh.2012.e7

1440

PDF: 535

HTML: 1294
Dark-field microscopy enhance visibility of CD31 endothelial staining

Eva Jennische, Stefan Lange, Ragnar Hultborn

01-07-2020

https://doi.org/10.4081/ejh.2020.3133

1498

PDF: 743

HTML: 10
Tumor cells-derived exosomal PD-L1 promotes the growth and invasion of lung cancer cells in vitro via mediating macrophages M2 polarization

Xiangjun Lu, Jian Shen, Siyuan Huang, Dongdong Liu, Haitao Wang

01-08-2023

https://doi.org/10.4081/ejh.2023.3784

1898

PDF: 854

HTML: 21
Evidence that muscle cells do not express the histidine-rich glycoprotein associated with AMP deaminase but can internalise the plasma protein

A.R.M. Sabbatini, L. Mattii, B. Battolla, E. Polizzi, D. Martini, M. Ranieri-Raggi, A.J.G. Moir, A. Raggi

e6

25-02-2011

https://doi.org/10.4081/ejh.2011.e6

1456

PDF: 535

HTML: 2616

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