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55 | A SELF-FOLDING HUMAN NEURAL TUBE MODEL USING 4D SMART SCAFFOLDS C. Dell’Amico1, I. Chiesa2, A. Toffano1, A. Esposito2, P. Mancini2, C. Magliaro2, A. Louvi3, C. De Maria2, M. Onorati1 | 1Department of Biology, Unit of Cell, Molecular, and Developmental Biology, University of Pisa, Italy; 2Department of Information Engineering and Research Center Enrico Piaggio, University of Pisa, Italy; 3Departments of Neurosurgery and Neuroscience, Yale School of Medicine, New Haven, USA
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P08 | COMBINED EFFECTS OF NONYLPHENOL AND STEROID HORMONES ON HUMAN PROSTATE CELL LINE S. Boccia1, A. Mileo2, F. Carrella3, R. Sciarrillo4, A. Capaldo1, M. De Falco5 | 1Dept. of Biology, University Federico II of Naples, Naples, Italy; 2Dept. of Medicine and Health Sciences, University of Molise, Campobasso, Italy; 3Dept. of Science and Technologies, University of Sannio, Benevento, Italy; 4Dept. of Science and Technologies, University of Sannio, Benevento, Italy; 5Dept. of Biology, University Federico II of Naples,; National Institute of Biostructures and Biosystems (INBB), Rome, Italy
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The penetration of methanol into bovine cardiac and hepatic tissues is faster than ethanol and formalin
2073PDF: 853Supplementary: 210HTML: 53 -
MICROBIAL INFLUENCE ON PERIPHERAL NERVOUS SYSTEM: INSIGHTS FROM GERM-FREE AND RECOLONIZED MICE Farzin S1, Pellegrino D1, Cicconetti C2, Bertone F1, Oliviero S2, Basic M3, Bolsega S3, Haastert-Talini K4, Cescon M5, Gambarotta G1 and Ronchi G1 | 1Department of Clinical and Biological Sciences (DSCB), University of Torino, Orbassano, (Torino), Italy; 2Department of Life Sciences and System Biology, University of Torino, Torino, Italy; 3Institute for Laboratory Animal Science and Central Animal Facility, Hannover Medical School, Hannover, Lower- Saxony, Germany; 4Institute of Neuroanatomy and Cell Biology, Hannover Medical School, Hannover, Lower-Saxony, Germany; 5Department of Molecular Medicine, University of Padova, Padova, Italy
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P81 | AN IN VITRO CYTOTOXIC, PROAPOPTOTIC AND ANTITUMOR ACTIVITY ASSESSMENT FOR CERAMIDASE INHIBITORS ON HUMAN LARYNGEAL CANCER CELLS C. Vejselova Sezer1, M. Cengiz2, B. Gür3, R. Dündar4, C. Cingi5, H.M. Kutlu6 | 1Department of Biology, Kutahya Dumlupinar University, Kutahya, Türkiye; 2Department of Mathematics and Physical Sciences Education, Siirt University, Siirt, Türkiye; 3Department of Biochemistry, Iğdır University, Iğdır, Türkiye; 4Department of Otorhinolaryngology, Acibadem Eskişehir Hospital, Eskişehir, Türkiye; 5Department of Otorhinolaryngology, Eskişehir Osmangazi University, Eskişehir, Türkiye; 6Department of Biology, Eskişehir Technical University, Eskişehir, Türkiye
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Epithelial expression of the hormone leptin by bovine skin
2071PDF: 1442HTML: 30 -
Management of autofluorescence in formaldehyde-fixed myocardium: choosing the right treatment
2058PDF: 1107Supplementary: 130HTML: 35 -
Transcription factors - Methods and protocols
951PDF: 451HTML: 150 -
THE LINK BETWEEN NEURONAL BRANCHING AND PAIN Dotta S1, Muraglia L1, Musso G1, Rother F2,3, Pritz. C1, Luzzati F1, Bader M2,3,4, Buffo A1, Vercelli A1 and Marvaldi L1 | 1Department of Neuroscience Rita Levi Montalcini, Neuroscience Institute Cavalieri Ottolenghi, University of Turin, Orbassano (TO), Italy; 2Max Delbrück Center for Molecular Medicine, Berlin, Germany; 3Center for Structural and Cellular Biology in Medicine, Institute of Biology, University of Lübeck, Germany; 4Charité, Universitätsmedizin, Berlin, Germany
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P86 | THE EFFECTS OF NICOTINIC ACETYLCHOLINE RECEPTOR INHIBITION ON THE EXPRESSION OF BRAIN DERIVED NEUROTROPHIC FACTOR IN THE OLFACTORY BULB ORGANOTYPIC SLICE CULTURES I. Atasoy1, S. Yilmazer2, D. Gezen Ak3, E. Dursun3 | 1Medical Biology Department, İstabul University-Cerrahpasa Faculty of Medicine Istanbul, Türkiye; 2Department of Medical Biology Halic University, Faculty of Medicine, Istanbul, Türkiye; 3Brain and Neurodegenerative Disorders Research Laboratories, Department of Neuroscience, Institute of Neurological Sciences, Istanbul University-Cerrahpasa, Istanbul, Türkiye
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64 | UNDERSTANDING PROPERTIES OF NEURAL STEM CELLS ISOLATED FROM A MONOGENIC MOUSE MODEL OF AUTISM EXPRESSING THE R451C NLGN3 MUTATION F. Salvadori, T. Diamanti, L. Santini, S. D’Angelo, F. Serafini, M. Fidaleo, G. Lupo, A. De Jaco, E. Cacci | Department of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, Italy
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P51 | DECELLULARIZATION OF HUMAN LUNG SCAFFOLD FOR TUMOR MICROENVIRONMENT MODELLING STUDY A. Mileo1, S. Boccia2, E. Del Genio1, R. Pastore1, M. De Falco3, I. Belviso4 | 1Dept. of Medicine and Health Sciences, University of Molise, Campobasso, Italy; 2Dept. of Biology, University of Naples Federico II, Naples, Italy; 3Dept. of Biology, University of Naples Federico II, Naples, Italy; National Institute of Biostructures and Biosystems (INBB), Rome, Italy; 4Dept. of Wellness, Nutrition and Sport, Telematic University Pegaso, Naples, Italy
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PARP-1 protein expression in glioblastoma multiforme
3374PDF: 1356HTML: 1819 -
31 | LOSS OF IMPG2 PARALOGS LEADS TO RETINAL DEGENERATION IN ZEBRAFISH DOUBLE KNOCK-OUT MODEL C. Fogliano1, A. Cumplido Mayoral2, L. Balasco2, S. Casarosa2|3, B. Avallone1 | 1Dept. of Biology, University of Naples Federico II, Italy 2Dept. of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Italy; 3Center for Medical Sciences, University of Trento, Italy
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P14 | THE ROLE OF CHROMATIN REORGANIZATION IN HYPOXIA ADAPTATION M. Cavallo1, C. Casali1, L. Giulini1, A. Dia1, D. Tunesi1, G. Milanesi1, G. Mazzini2, M. Biggiogera1 | 1Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, Pavia; Italy; 2Institute of Molecular Genetics, National Research Council (IGMCNR), Pavia, Italy
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P28 | EFFECTS OF CHRONIC LOW-TEMPERATURE EXPOSURE ON THE BEHAVIOUR OF ADULT ZEBRAFISH (DANIO RERIO) A.S. Sanchez Garcia1, F. Frabetti2, M. Toni1 | 1Dep. of Biology and Biotechnologies “Charles Darwin”, Sapienza University, Rome, Italy; 2Dep. of Medical and Surgical Sciences—DIMEC, University of Bologna, Bologna, Italy
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INVESTIGATION OF STRUCTURAL PRESERVATION OF PERIPHERAL NERVE EXTRACELLULAR MATRIX FOLLOWING EX VIVO LIMB PERFUSION Metafune M1,2, Haastert-Talini K3, Muratori L1,2 and Raimondo S1,2 | 1Department of Clinical and Biological Sciences, Neuroscience Institute Cavalieri Ottolenghi (NICO), University of Turin, Turin, Italy; 2Institute of Neuroanatomy and Cell Biology, Hannover Medical School, Hannover, Lower-Saxony, Germany
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Identification of abnormally high expression of POGZ as a new biomarker associated with a poor prognosis in osteosarcoma
2040PDF: 750Supplementary: 154HTML: 31 -
17 | EXPLORING THE FUNCTIONAL ROLE OF THE PROTEOGLYCAN ESM1 IN THE BREAST CANCER TUMOR MICROENVIRONMENT E. Peri1, S. Feo1|2, E. Roz3, A. D’Ascola4, P. Cancemi1|2 | 1Department of Biological Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Italy; 2Experimental Center of Onco Biology (COBS), Palermo, Italy; 3La Maddalena Hospital III Level Oncological Department, Palermo, Italy; 4Department of Clinical and Experimental Medicine, University of Messina, Italy
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LEVERAGING ORGANOID TECHNOLOGIES TO CHARACTERIZE DYNAMIC TUMOR CELL STATES DRIVING TREATMENT RESISTANCE IN PANCREATIC CANCER A. Papargyriou1-6, M. Najajreh1-4, D.P. Cook7, C.H. Maurer1,2, S. Bärthel8, P. Putze8, H.A. Messal9, S.K. Ravichandran1-3, T. Richter10, M. Knolle11, T. Metzler12, A.R. Shastri1,2,3, R. Öllinger13, J. Jasper1,2,3, L. Schmidleitner1,2,3, C.Schneeweis8, H. Ishikawa-Ankerhold14, T. Engleitner13, S. Dobiasch15, I. Heid, M.D. Luecken16, K. Steiger12, G. Kaissis11, J. Van Rheenen9, F.J. Theis10, D. Saur8, R. Rad13, M. Reichert1,2,3,4,5,17,18 | 1Translational Pancreatic Cancer Research Center, Klinik und Poliklinik für Innere Medizin II, Klinikum rechts der Isar, Technical University of Munich, Germany; 2Klinik und Poliklinik für Innere Medizin II, Klinikum rechts der Isar, Technical University of Munich, Germany; 3Center for Functional Protein Assemblies, Technical University of Munich, Garching, Germany; 4Center for Organoid Systems (COS), Technical University of Munich, Garching, Germany; 5Bavarian Cancer Research Center (BZKF), Munich, Germany; 6Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg, Germany; 7University of Ottawa, Faculty of Medicine, Department of Cellular and Molecular Medicine, Ottawa, ON, Canada; 8Chair for Translational Cancer Research and Institute of Experimental Cancer Therapy, Klinikum rechts der Isar, School of Medicine, Technical University of Munich, Germany; 9Division of Molecular Pathology, Oncode Institute, The Netherlands Cancer Institute, Plesmanlaan 121, 1066CX, Amsterdam, The Netherlands. 10Institute of Computational Biology, Helmholtz Center Munich, Neuherberg, Germany; 11Institute of Diagnostic and Interventional Radiology, Klinikum rechts der Isar München, Technical University of Munich, Germany; 12Comparative Experimental Pathology, Institut für Allgemeine Pathologie und Pathologische Anatomie, School of Medicine, Technical University of Munich, Germany; 13Institute of Molecular Oncology and Functional Genomics, School of Medicine, Technical University of Munich, Germany; 14Department of Medicine I, University Hospital of the LudwigMaximilians-University Munich, Germany; 15Department of Radiation Oncology, Technical University of Munich, Germany; 16Institute of Lung Health and Immunity (LHI), Helmholtz Munich, Comprehensive Pneumology Center (CPC-M), Germany; Member of the German Center for Lung Research (DZL); 17German Cancer Consortium (DKTK), Munich, Germany; 18Munich Institute of Biomedical Engineering (MIBE), Technical University of Munich, Germany
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65 | EFFECTS OF ENVIRONMENTAL TEMPERATURE VARIATION AND BDNF MODULATION ON THE SPATIO-TEMPORAL SHOALING BEHAVIOUR OF ADULT ZEBRAFISH (DANIO RERIO) REVEALED BY TWO- AND THREE-DIMENSIONAL ANALYSIS A.S. Sanchez Garcia1, F. Frabetti2, G. Brighi2, G. Tedeschi3|4, A. Racca5, E. Alleva5, M. Toni1 | 1Dept. of Biology and Biotechnologies “Charles Darwin”, Rome, Italy; 2Dept. of Medical and Surgical Sciences, Bologna, Italy; 3Dept. of Veterinary Medicine and Animal Science, University of Milan, Italy; 4CRC “Innovation for Well-Being and Environment”, University of Milan, Italy; 5Centre for Behavioural Sciences and Mental Health, Rome, Italy
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INSIGHTS INTO THE EVOLUTION OF DOPAMINERGIC CONTROL OF REPRODUCTION: THE BRAIN-PITUITARY-GONADAL AXIS OF THE SMALL-SPOTTED CATSHARK SCYLIORHINUS CANICULA Porceddu R1,2, Mulas G1, Porcu C1 and Follesa MC1,2 | 1Sezione di Biologia Animale ed Ecologia, Dipartimento di Scienze della Vita e dell’Ambiente, Università degli Studi di Cagliari, Cagliari, Italy; 2CoNISMa Consorzio Nazionale Interuniversitario per le Scienze Mare, Rome, Italy
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PO54 | FATTY INFILTRATION AND POTENTIAL ADIPOGENIC SHIFT OF SKELETAL MUSCLE CELLS: MULTIPARAMETRIC HISTOLOGICAL ANALYSIS OF MYOSTEATOSIS L. Morello1, G. Stati1, V. Giansante1, S. Lattanzio1, P. Cerritelli1, D. Bruni1, S. Cinti2, R. Di Pietro1,3,4 | 1Department of Medicine and Aging Sciences, “G. d’ Annunzio” University of Chieti-Pescara, Chieti, Italy; 2Department of Experimental and Clinical Medicine, Center of Obesity, Polytechnic University of Marche, Ancona, Italy; 3StemTeCh Group, G. d’ Annunzio Foundation, “G. d’ Annunzio” University of Chieti Pescara, Chieti, Italy; 4Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, Department of Biology, College of Science and Technology, Temple University, Philadelphia, PA, USA
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04 | MODULATION OF NEUROINFLAMMATION IN IL-1BETA-INDUCED HUMAN ASTROCYTES: EFFECTS OF GREEN LEAVES AND RHIZOMES EXTRACT FROM POSIDONIA OCEANICA (L.) DELILE G. Abruscato1, L. Sileo2, G. Scanavino2, C. Guerriero2, M. Mauro1, M. Vazzana1|3, V. Arizza1|3, C. Luparello1|3, A.M.Tata2 | 1Dept. of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Italy; 2Dept. of Biology and Biotechnologies Charles Darwin, Sapienza- University of Rome, Italy; 3National Biodiversity Future Center (NBFC), Italy
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Adipose-derived stem cells promote the recovery of intestinal barrier function by inhibiting the p38 MAPK signaling pathway
1893PDF: 613Supplementary Figure: 95HTML: 61 -
68 | RESILIENCE ASSOCIATED WITH DEFECTS IN VITAMIN B12 METABOLISM: BALANCING BETWEEN HOMEOSTASIS AND DISEASE E. Selita1, E. Tomaselli2, G. Di Matteo3, L. Mannina3, M. Rossi2|4, D. Passeri2|4, L. Angeloni2|4, M. Fidaleo1|4 | 1Dept of Biology and Biotechnology, Charles Darwin, University of Rome Sapienza, Italy; 2Dept of Basic and Applied Sciences for Engineering, Sapienza University of Rome, Italy; 3Dept of Chemistry and Technology of Drugs, Sapienza University of Rome, Italy; 4Research Centre for Nanotechnology for Engineering of Sapienza (CNIS), Sapienza University of Rome, Italy
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P15 | PHB/JAK/H3Y41PH AXIS REGULATES MEIOTIC RECOMBINATION IN SPERMATOGENESIS H. Chen1, L. Zhang1, W. Tan-Tai1, M. Liu2, H. Shi3, W. O4, P.A. Martin-DeLeon5 | 1Department of Anatomy, Histology & Embryology, Shanghai Medical College, Fudan University, Shanghai, China; 2State Key Laboratory of Molecular Biology, Chinese Academy of Sciences-University of Chinese Academy of Sciences, Shanghai, China; 3NHC Key Lab of Reproduction Regulation, Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai, China; 4School of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, China; 5Department of Biological Sciences, University of Delaware, Newark, USA
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Transgenic mouse - Methods and protocols, 2nd edition
1388PDF: 499HTML: 240 -
THE EFFECT OF ANTIBIOTIC-INDUCED DYSBIOSIS ON PERIPHERAL NERVE REPAIR Pellegrino D1, El Soury M1, Farzin S1, Elmelgy AA1, Muratori L1, Calabrò S2, Ferrocino I3, Ferrero G1, Camandona A1, Haastert- Talini K4, Cescon M2, Gambarotta G1 and Ronchi G1 | 1Department of Clinical and Biological Sciences & Neuroscience Institute Cavalieri Ottolenghi (NICO), University of Torino, Orbassano, (Torino), Italy; 2Department of Molecular Medicine, University of Padova, Padova, Italy; 3Department of Scienze Agrarie, Forestali e Alimentari, DISAFA, University of Torino, Grugliasco, (Torino), Italy; 4Institute of Neuroanatomy and Cell Biology, Hannover Medical School, Carl-Neuberg-Str.1, Hannover, Lower-Saxony, Germany
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RNA therapeutics - Function, design and delivery
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Two-dimensional electrophoresis protocols
783PDF: 450HTML: 166 -
P05 | COMBINED EMBRYOTOXIC EFFECTS OF MICROPLASTICS AND HEAVY METALS IN XENOPUS LAEVIS S. Di Marino, C. Fogliano, C.M. Motta, B. Avallone, R. Carotenuto | Dept. of Biology, University of Naples Federico II, Italy
70PDF: 24 -
16 | EXTRACELLULAR VESICLES RELEASED BY CUMULUS CELLS CONTRIBUTE TO THE ACQUISITION OF MOUSE OOCYTE’S DEVELOPMENTAL COMPETENCE Giulia Brazzale1, Mario Zanoni1, Valeria Merico1, Giulia Fiorentino1, Paola Rebuzzini1, Andrea Fantinato1, Margherita Isernia1, Sergio Comincini1, Danilo Cimadomo1|2, Maurizio Zuccotti1 | 1Dept. of Biology and Biotechnology, University of Pavia, Italy; 2IVIRMA Global Research Alliance, IVIRMA Italia, Rome, Italy
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ROLE OF MITOCHONDRIAL METABOLISM AND CONTACT SITES IN CANCER CELL DEATH AND SURVIVAL F. Severin, A. Ruzza, F. Vianello, E. Zaltron, I. Celotti, M. Scavezzon, S. Toffanin, C. Bastianello, L. Leanza | Department of Biology, University of Padova, Padova, Italy
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P03 | ALTERED NEUROTRANSMISSION AND METABOLISM IN A GFAP R239C ZEBRAFISH MODEL OF ALEXANDER DISEASE S. Candiani1|2, M. Bozzo1, V. Bazzurro1, D. Bellitto3, A. Tripi1, S. Ferrando1, G. Rosa1, T. Bacchetti3 | 1Dept. of Biology, University of Genova, Italy; 2RCCS Azienda Ospedaliera Metropolitana, Genova, Italy; 3UO Proteomica e Spettrometria di Massa, IRCCS Ospedale Policlinico San Martino, Genova, Italy
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EX VIVO MAPPING OF NIGRO-THALAMIC DOPAMINERGIC TERMINALS REVEALS REGIONAL SPECIALIZATION AND SYNAPTIC MARKERS IN THE HUMAN THALAMUS Cirillo G1, Montella M2, La Mantia E2, Tedesco I2, De Luca C1, Virtuoso A1, De Angelis F1, Riccio L1, Esposito F3, Cirillo M3, Franco R2 and Papa M1 | 1Division of Human Anatomy, Neuronal Networks Morphology & Systems Biology Lab, Department of Mental and Physical Health and Preventive Medicine, University of Campania “Luigi Vanvitelli, Naples, Italy; 2Division of Pathology, Department of Mental and Physical Health and Preventive Medicine, University of Campania “Luigi Vanvitelli, Naples, Italy; 3Department of Advanced Medical and Surgical Sciences, Advanced MRI Research Center, University of Campania Luigi Vanvitelli, Naples, Italy
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